Faculty of Veterinary Medicine, University of Belgrade, Bulevar oslobođenja 18, 11000 Belgrade, Serbia.
Faculty of Veterinary Medicine, University of Belgrade, Bulevar oslobođenja 18, 11000 Belgrade, Serbia.
Ticks Tick Borne Dis. 2017 Oct;8(6):907-914. doi: 10.1016/j.ttbdis.2017.07.009. Epub 2017 Aug 2.
Babesia canis and Dirofilaria immitis are emerging and geographically overlapping vector-borne pathogens in dogs. Infection with B. canis leads to acute-phase response (APR) that can be mild to severe and results in either non-complicated or complicated forms of the disease. The aim of this study was to determine whether acute B. canis infection is more severe in dogs with underlying asymptomatic D. immitis infection. Dogs of both sexes, different ages and breeds, with naturally occurring mono-infections with B. canis (n=13) and D. immitis (n=18) and co-infected dogs (n=7) were enrolled as well as healthy controls (n=15). Routine haematology and biochemistry, agarose gel electrophoresis (agEF) protein fraction separation and enzyme-linked immunosorbent assay (ELISA) for serum amyloid A (SAA) were performed. Based on clinical and laboratory findings, sepsis was diagnosed in the majority of dogs with acute B. canis infection with or without underlying asymptomatic D. immitis infection. Overall, haematology, biochemistry and agEF pattern changes were induced and dictated by acute B. canis infection whether or not the dogs had an asymptomatic D. immitis infection. D. immitis infection slightly influenced the level of anaemia, slightly aggravated the level of dehydration and increased the concentration of γ-globulins in acute-phase B. canis infection. D. immitis infection prevented B. canis-induced leukopenia. SAA equally increased in dogs with acute B. canis infection with or without underlying D. immitis infection. The level of SAA was not changed in dogs with asymptomatic D. immitis when compared to the controls. In conclusion, asymptomatic D. immitis infection does not influence overall APR after acute B. canis infection.
犬巴贝斯虫和犬心丝虫是新兴的、地理上重叠的犬类媒介传播病原体。感染犬巴贝斯虫会导致急性期反应（APR），其严重程度可从轻度到重度，并导致疾病出现非复杂性或复杂性形式。本研究旨在确定在患有隐性无症状犬心丝虫感染的犬中，急性犬巴贝斯虫感染是否更为严重。本研究纳入了自然发生的单重感染犬巴贝斯虫（n=13）和犬心丝虫（n=18）以及混合感染犬（n=7）以及健康对照组（n=15）的雄性和雌性、不同年龄和品种的犬。进行了常规血液学和生物化学、琼脂糖凝胶电泳（agEF）蛋白部分分离和酶联免疫吸附试验（ELISA）检测血清淀粉样蛋白 A（SAA）。根据临床和实验室发现，在大多数患有急性犬巴贝斯虫感染的犬中诊断出败血症，无论是否存在隐性无症状犬心丝虫感染。总的来说，急性犬巴贝斯虫感染会导致血液学、生物化学和 agEF 模式的变化，无论犬是否患有隐性无症状犬心丝虫感染。犬心丝虫感染略微影响贫血程度，略微加重脱水程度，并增加急性犬巴贝斯虫感染时的γ-球蛋白浓度。犬心丝虫感染可预防犬巴贝斯虫诱导的白细胞减少症。急性犬巴贝斯虫感染的犬中 SAA 同等增加，无论是否存在隐性犬心丝虫感染。与对照组相比，无症状犬心丝虫感染的犬的 SAA 水平没有变化。结论，隐性犬心丝虫感染不会影响急性犬巴贝斯虫感染后的总体 APR。
