Méndez J C, Carretón E, Martínez S, Tvarijonaviciute A, Cerón J J, Montoya-Alonso J A
Internal Medicine, Faculty of Veterinary Medicine, University of Las Palmas de Gran Canaria, 35413 Arucas, Las Palmas, Spain.
Interdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Veterinary School, Campus of Excellence Mare Nostrum, University of Murcia, Espinardo, 30100 Murcia, Spain.
Vet Parasitol. 2014 Aug 29;204(3-4):420-5. doi: 10.1016/j.vetpar.2014.05.016. Epub 2014 May 17.
The aim of this study was to determine concentrations of different positive and negative acute phase proteins (C-reactive protein, haptoglobin, albumin and paraoxonase-1) in dogs naturally infected with Dirofilaria immitis at the time of diagnosis. 194 dogs were included in the study. All were evaluated for the presence or absence of D. immitis circulating antigens and for the presence or absence of microfilariae and a clinical examination was carried out. 38 dogs were negative and 156 dogs were positive for circulating D. immitis antigens. A significant increase in C-reactive protein and significant decreases in albumin and paraoxonase-1 activity were observed in positive dogs. These changes appeared with independence of the presence/absence of microfilariae or clinical signs. C-reactive protein was the only acute phase protein that showed significant differences between asymptomatic and symptomatic dogs. Interestingly, the increases seen in C-reactive protein values were not accompanied by increases in haptoglobin, and haptoglobin even decreased in the dogs with microfilaria. This could be due to the hemolytic anemia which can be produced in dirofilariasis. In conclusion, there is an acute phase response (with increases in C-reactive protein and decreases in albumin and paraoxonase-1) and a divergence in the behaviour between C-reactive protein and haptoglobin in dogs with D. immitis.
本研究的目的是确定在诊断时自然感染犬恶丝虫的犬只体内不同正负急性期蛋白（C反应蛋白、触珠蛋白、白蛋白和对氧磷酶-1）的浓度。194只犬被纳入研究。所有犬只均接受了犬恶丝虫循环抗原的有无以及微丝蚴的有无评估，并进行了临床检查。38只犬循环犬恶丝虫抗原呈阴性，156只犬呈阳性。在阳性犬中观察到C反应蛋白显著升高，白蛋白和对氧磷酶-1活性显著降低。这些变化与微丝蚴的有无或临床症状无关。C反应蛋白是唯一在无症状犬和有症状犬之间表现出显著差异的急性期蛋白。有趣的是，C反应蛋白值升高并未伴随触珠蛋白升高，甚至在有微丝蚴的犬只中触珠蛋白还降低了。这可能是由于恶丝虫病可导致的溶血性贫血。总之，感染犬恶丝虫的犬只存在急性期反应（C反应蛋白升高，白蛋白和对氧磷酶-1降低），且C反应蛋白和触珠蛋白的行为存在差异。
