Instituto de Investigaciones Agrobiológicas de Galicia (IIAG), Consejo Superior de Investigaciones Científicas (CSIC), Aptdo. 122, Santiago de Compostela 15780, Spain.
Instituto de Investigaciones Agrobiológicas de Galicia (IIAG), Consejo Superior de Investigaciones Científicas (CSIC), Aptdo. 122, Santiago de Compostela 15780, Spain.
Environ Pollut. 2017 Dec;231(Pt 1):237-251. doi: 10.1016/j.envpol.2017.07.097. Epub 2017 Aug 10.
Gentle remediation options (GRO) are based on the combined use of plants, associated microorganisms and soil amendments, which can potentially restore soil functions and quality. We studied the effects of three GRO (aided-phytostabilisation, in situ stabilisation and phytoexclusion, and aided-phytoextraction) on the soil microbial biomass and respiration, the activities of hydrolase enzymes involved in the biogeochemical cycles of C, N, P, and S, and bacterial community structure of trace element contaminated soils (TECS) from six field trials across Europe. Community structure was studied using denaturing gradient gel electrophoresis (DGGE) fingerprinting of Bacteria, α- and β-Proteobacteria, Actinobacteria and Streptomycetaceae, and sequencing of DGGE bands characteristic of specific treatments. The number of copies of genes involved in ammonia oxidation and denitrification were determined by qPCR. Phytomanagement increased soil microbial biomass at three sites and respiration at the Biogeco site (France). Enzyme activities were consistently higher in treated soils compared to untreated soils at the Biogeco site. At this site, microbial biomass increased from 696 to 2352 mg ATP kg soil, respiration increased from 7.4 to 40.1 mg C-CO kg soil d, and enzyme activities were 2-11-fold higher in treated soils compared to untreated soil. Phytomanagement induced shifts in the bacterial community structure at both, the total community and functional group levels, and generally increased the number of copies of genes involved in the N cycle (nirK, nirS, nosZ, and amoA). The influence of the main soil physico-chemical properties and trace element availability were assessed and eventual site-specific effects elucidated. Overall, our results demonstrate that phytomanagement of TECS influences soil biological activity in the long term.
温和修复选项（GRO）基于植物、相关微生物和土壤改良剂的联合使用，这些方法有可能恢复土壤功能和质量。我们研究了三种 GRO（辅助植物稳定化、原位稳定化和植物隔离，以及辅助植物提取）对欧洲六个田间试验中受微量元素污染土壤（TECS）的土壤微生物生物量和呼吸作用、涉及 C、N、P 和 S 生物地球化学循环的水解酶活性以及细菌群落结构的影响。使用变性梯度凝胶电泳（DGGE）指纹图谱对细菌、α-和β-变形菌、放线菌和链霉菌科进行了群落结构研究，并对特定处理特征的 DGGE 条带进行了测序。通过 qPCR 确定参与氨氧化和反硝化的基因的拷贝数。在三个地点，植物管理增加了土壤微生物生物量，在 Biogeco 地点（法国）增加了呼吸作用。与未处理的土壤相比，处理过的土壤中的酶活性始终更高。在该地点，微生物生物量从 696 增加到 2352 mg ATP kg 土壤，呼吸作用从 7.4 增加到 40.1 mg C-CO kg 土壤 d，与未处理土壤相比，处理过的土壤中的酶活性增加了 2-11 倍。植物管理导致了总群落和功能组水平的细菌群落结构发生变化，并普遍增加了参与 N 循环的基因（nirK、nirS、nosZ 和 amoA）的拷贝数。评估了主要土壤理化性质和微量元素可用性的影响，并阐明了可能的特定地点的影响。总体而言，我们的研究结果表明，长期以来，TECS 的植物管理会影响土壤生物活性。
