Sun Mitchell Y, Geyer Melissa, Komarova Yulia A
Department of Pharmacology and The Center for Lung and Vascular Biology, University of Illinois College of Medicine, Chicago, IL, 60612, USA.
Cell Mol Life Sci. 2017 Nov;74(22):4189-4207. doi: 10.1007/s00018-017-2624-8. Epub 2017 Aug 12.
The endothelium, a monolayer of endothelial cells lining vessel walls, maintains tissue-fluid homeostasis by restricting the passage of the plasma proteins and blood cells into the interstitium. The ion Ca, a ubiquitous secondary messenger, initiates signal transduction events in endothelial cells that is critical to control of vascular tone and endothelial permeability. The ion Ca is stored inside the intracellular organelles and released into the cytosol in response to environmental cues. The inositol 1,4,5-trisphosphate (IP) messenger facilitates Ca release through IP receptors which are Ca-selective intracellular channels located within the membrane of the endoplasmic reticulum. Binding of IP to the IPRs initiates assembly of IPR clusters, a key event responsible for amplification of Ca signals in endothelial cells. This review discusses emerging concepts related to architecture and dynamics of IPR clusters, and their specific role in propagation of Ca signals in endothelial cells.
内皮是一层衬于血管壁的内皮细胞，通过限制血浆蛋白和血细胞进入间质来维持组织液稳态。离子钙作为一种普遍存在的第二信使，在内皮细胞中启动信号转导事件，这对控制血管张力和内皮通透性至关重要。离子钙储存在细胞内细胞器中，并根据环境信号释放到细胞质中。肌醇1,4,5-三磷酸（IP）信使通过IP受体促进钙释放，IP受体是位于内质网膜内的钙选择性细胞内通道。IP与IP受体的结合启动IP受体簇的组装，这是在内皮细胞中负责钙信号放大的关键事件。本综述讨论了与IP受体簇的结构和动力学相关的新观点，以及它们在内皮细胞钙信号传播中的特定作用。
