用于免洗活细胞染色和成像的基于硼二吡咯亚甲基的染料。

BODIPY-based dye for no-wash live-cell staining and imaging.

作者信息

Pakhomov Alexey A, Deyev Igor E, Ratnikova Natalia M, Chumakov Stepan P, Mironiuk Veronika B, Kononevich Yuriy N, Muzafarov Aziz M, Martynov Vladimir I

机构信息

M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russian Federation.

A.N. Nesmeyanov Institute of Organoelement Compounds, Moscow, Russian Federation.

出版信息

Biotechniques. 2017 Aug 1;63(2):77-80. doi: 10.2144/000114577.

DOI:10.2144/000114577

PMID:28803543

Abstract

In nonpolar solvents, hydrophobic organic fluorophores often show bright fluorescence, whereas in polar media, they usually suffer from aggregation-caused quenching (ACQ). Here, we harnessed this solvatochromic behavior of a 1,3,5,7-tetramethyl-BODIPY derivative for cell staining and applied it to live-cell imaging and flow cytometry. As opposed to commercially available dyes, this BODIPY derivative showed excellent contrast immediately after staining and did not require any wash-off.

摘要

在非极性溶剂中，疏水性有机荧光团通常会发出明亮的荧光，而在极性介质中，它们通常会因聚集导致猝灭（ACQ）。在此，我们利用1,3,5,7-四甲基-BODIPY衍生物的这种溶剂化显色行为进行细胞染色，并将其应用于活细胞成像和流式细胞术。与市售染料不同，这种BODIPY衍生物在染色后立即显示出优异的对比度，并且不需要任何洗脱。