Gou Chenyu, Liu Xiangzhen, Shi Xiaomei, Chai Hanjing, He Zhi-Ming, Huang Xuan, Fang Qun
Department of Obstetrics and Gynecology,Second Affiliated Hospital of Guangzhou Medical University,Guangzhou,China.
Department of Oral and Maxillofacial Surgery,First Affiliated Hospital of Sun Yat-Sen University,Guangzhou,China.
Twin Res Hum Genet. 2017 Oct;20(5):389-394. doi: 10.1017/thg.2017.41. Epub 2017 Aug 14.
CDKN1C and KCNQ1OT1 are imprinted genes that might be potential regulators of placental development. This study investigated placental expressions of CDKN1C and KCNQ1OT1 in monozygotic twins with and without selective intrauterine growth restriction (sIUGR). Seventeen sIUGR and fifteen normal monozygotic(MZ) twin pairs were examined. Placental mRNA expressions of CDKN1C and KCNQ1OT1 were detected by real-time fluorescent quantitative PCR. CDKN1C protein expression was detected by immunohistochemical assay and Western-blotting. In the sIUGR group, smaller fetuses had a smaller share of the placenta, and CDKN1C protein expression was significantly increased while KCNQ1OT1 mRNA expression was significantly decreased. The CDKN1C/KCNQ1OT1 mRNA ratio was lower in the larger fetus than in the smaller fetus (p < .05). In the control group, CDKN1C protein expression showed no difference between larger and smaller fetuses, while KCNQ1OT1 mRNA expression was significantly lower in the larger fetus, and the CDKN1C/KCNQ1OT1 mRNA ratio was higher in the larger fetus than in the smaller fetus (p < .05). Our findings showed that pathogenesis of sIUGR may be related to the co-effect of the up-regulated protein expression of CDKN1C and down-regulated mRNA expression of KCNQ1OT1 in the placenta.
CDKN1C和KCNQ1OT1是印记基因，可能是胎盘发育的潜在调节因子。本研究调查了患有和未患有选择性宫内生长受限（sIUGR）的单卵双胞胎中CDKN1C和KCNQ1OT1的胎盘表达情况。研究了17对sIUGR单卵双胞胎和15对正常单卵双胞胎。通过实时荧光定量PCR检测CDKN1C和KCNQ1OT1的胎盘mRNA表达。通过免疫组织化学分析和蛋白质印迹法检测CDKN1C蛋白表达。在sIUGR组中，较小的胎儿在胎盘中所占份额较小，CDKN1C蛋白表达显著增加，而KCNQ1OT1 mRNA表达显著降低。较大胎儿的CDKN1C/KCNQ1OT1 mRNA比值低于较小胎儿（p < 0.05）。在对照组中，较大和较小胎儿之间的CDKN1C蛋白表达无差异，而较大胎儿的KCNQ1OT1 mRNA表达显著较低，较大胎儿的CDKN1C/KCNQ1OT1 mRNA比值高于较小胎儿（p < 0.05）。我们的研究结果表明，sIUGR的发病机制可能与胎盘中CDKN1C蛋白表达上调和KCNQ1OT1 mRNA表达下调的共同作用有关。
