Department of Theoretical Bioinformatics, German Cancer Research Center (DKFZ), Germany; Heidelberg Center for Personalized Oncology, DKFZ-HIPO, DKFZ, Heidelberg, Germany.
Department of Theoretical Bioinformatics, German Cancer Research Center (DKFZ), Germany.
J Biotechnol. 2017 Nov 10;261:53-62. doi: 10.1016/j.jbiotec.2017.08.006. Epub 2017 Aug 10.
The One Touch Pipeline (OTP) is an automation platform managing Next-Generation Sequencing (NGS) data and calling bioinformatic pipelines for processing these data. OTP handles the complete digital process from import of raw sequence data via alignment of sequencing reads to identify genomic events in an automated and scalable way. Three major goals are pursued: firstly, reduction of human resources required for data management by introducing automated processes. Secondly, reduction of time until the sequences can be analyzed by bioinformatic experts, by executing all operations more reliably and quickly. Thirdly, storing all information in one system with secure web access and search capabilities. From software architecture perspective, OTP is both information center and workflow management system. As a workflow management system, OTP call several NGS pipelines that can easily be adapted and extended according to new requirements. As an information center, it comprises a database for metadata information as well as a structured file system. Based on complete and consistent information, data management and bioinformatic pipelines within OTP are executed automatically with all steps book-kept in a database.
One Touch Pipeline（OTP）是一个自动化平台，用于管理下一代测序（NGS）数据，并调用生物信息学管道来处理这些数据。OTP 以自动化和可扩展的方式处理从原始测序数据导入到通过对齐测序读取来识别基因组事件的完整数字流程。主要追求三个目标：首先，通过引入自动化流程，减少数据管理所需的人力资源。其次，通过更可靠和快速地执行所有操作，减少生物信息学专家分析序列所需的时间。第三，通过安全的 Web 访问和搜索功能，在一个系统中存储所有信息。从软件架构的角度来看，OTP 既是信息中心，也是工作流管理系统。作为工作流管理系统，OTP 调用多个 NGS 管道，这些管道可以根据新的要求轻松进行适配和扩展。作为信息中心，它包含一个元数据信息数据库和一个结构化文件系统。基于完整一致的信息，OTP 中的数据管理和生物信息学管道会自动执行，所有步骤都在数据库中记录。
