Pyke K W, Papaoiannou J
Thymus. 1986;8(6):369-75.
Adult mouse bone marrow showed a reproducible scatter profile on low angle vs. right angle scatter by flow cytometry and it was possible to sort cells from different regions of the profile. The colonization of the third pharyngeal pouch in vitro was used as a test of thymocyte precursors. Alleles of the Thy-1 surface antigen, as well as the nuclear chromatin pattern of the ichthyosis mutant, were used to monitor chimerism. Thymus-colonizing cells were found in one region of the scatter profile, and these cells were negative for Thy-1 and Ia markers.
成年小鼠骨髓在流式细胞仪检测的低角度与直角散射中呈现出可重复的散射图谱，并且能够从图谱的不同区域分选细胞。将第三咽囊在体外的定植用作胸腺细胞前体的检测。Thy-1表面抗原的等位基因以及鱼鳞病突变体的核染色质模式用于监测嵌合体。在散射图谱的一个区域中发现了胸腺定植细胞，这些细胞的Thy-1和Ia标记呈阴性。
