Hvas Anne-Mette, Favaloro Emmanuel J
Aarhus University Hospital, Palle Juul-Jensens Boulevard 99, 8200, Aarhus N, Denmark.
Haematology Department, Sydney Centres for Thrombosis and Haemostasis, Institute of Clinical Pathology and Medical Research (ICPMR), Westmead Hospital, NSW Health Pathology, Westmead, NSW, 2145, Australia.
Methods Mol Biol. 2017;1646:321-331. doi: 10.1007/978-1-4939-7196-1_25.
Analysis of platelet function is widely used for diagnostic work-up in patients with increased bleeding tendency. During the last decades, platelet function testing has also been introduced for evaluation of antiplatelet therapy, but this is still recommended for research purposes only. Platelet function can also be assessed for hyper-aggregability, but this is less often evaluated. Light transmission aggregometry (LTA) was introduced in the early 1960s and has since been considered the gold standard. This optical detection system is based on changes in turbidity measured as a change in light transmission, which is proportional to the extent of platelet aggregation induced by addition of an agonist. LTA is a flexible method, as different agonists can be used in varying concentrations, but performance of the test requires large blood volumes and experienced laboratory technicians as well as specialized personal to interpret results. In the present chapter, a protocol for LTA is described including all steps from pre-analytical preparation to interpretation of results.
血小板功能分析广泛应用于出血倾向增加患者的诊断检查。在过去几十年中，血小板功能检测也被用于评估抗血小板治疗，但目前仍仅推荐用于研究目的。血小板功能也可针对高聚集性进行评估，但这种情况较少被评估。光透射聚集法（LTA）于20世纪60年代初被引入，此后一直被视为金标准。这种光学检测系统基于浊度的变化，通过光透射的变化来测量，其与添加激动剂诱导的血小板聚集程度成正比。LTA是一种灵活的方法，因为可以使用不同浓度的不同激动剂，但该检测的实施需要大量血液样本、经验丰富的实验室技术人员以及专业人员来解读结果。在本章中，将描述LTA的操作流程，包括从分析前准备到结果解读的所有步骤。
