Favaloro Emmanuel J, Mohammed Soma, Patzke Jürgen
Haematology Department, Sydney Centres for Thrombosis and Haemostasis, Institute of Clinical Pathology and Medical Research (ICPMR), Westmead Hospital, NSW Health Pathology, Westmead, NSW, 2145, Australia.
Haematology Department, Institute of Clinical Pathology and Medical Research (ICPMR), NSW Health Pathology, Westmead Hospital, Westmead, NSW, 2145, Australia.
Methods Mol Biol. 2017;1646:403-416. doi: 10.1007/978-1-4939-7196-1_30.
von Willebrand disease (VWD) is reportedly the most common inherited bleeding disorder and can also arise as an acquired syndrome (AVWS). These disorders arise due to defects and/or deficiency of the plasma protein von Willebrand factor (VWF). Laboratory testing for the VWF-related disorders requires assessment of both VWF level and VWF activity, the latter requiring multiple assays because of the many functions carried out by VWF to help prevent bleeding. The current paper describes protocols for assessment of VWF level by means of VWF antigen (VWF:Ag). These assays identify VWF levels by quantitative assessment of VWF protein by means of immunological assays. The most commonly performed assays for VWF:Ag comprise enzyme-linked immunosorbent assays (ELISA) and latex-enhanced immunoassays (LIA), as described in this chapter.
据报道，血管性血友病（VWD）是最常见的遗传性出血性疾病，也可作为一种获得性综合征（AVWS）出现。这些疾病是由于血浆蛋白血管性血友病因子（VWF）的缺陷和/或缺乏引起的。对VWF相关疾病的实验室检测需要评估VWF水平和VWF活性，由于VWF为预防出血发挥多种功能，所以对VWF活性的检测需要多种检测方法。本文描述了通过VWF抗原（VWF:Ag）评估VWF水平的方案。这些检测方法通过免疫检测法定量评估VWF蛋白来确定VWF水平。本章所述，最常用的VWF:Ag检测方法包括酶联免疫吸附测定（ELISA）和乳胶增强免疫测定（LIA）。
