Rocha Arrieta Y C, Rojas M, Vasquez G, Lopez J
Grupo de Inmunodeficiencias Primarias/Lab 530-531, Facultad de Medicina, Universidad de Antioquia UdeA, Medellín, Colombia.
Unidad de Citometría/Lab 420, Instituto de Investigaciones Médicas, Facultad de Medicina, Universidad de Antioquia UdeA, Medellín, Colombia.
Scand J Immunol. 2017 Oct;86(4):229-238. doi: 10.1111/sji.12592.
The release of DNA into the extracellular milieu by neutrophil during a process called NETosis has been postulated as an additional source of autoantigens; a process believed to be important in the pathogenesis of some autoimmune disease, such as systemic lupus erythematosus (SLE). However, it is not established if the B and T cells undergo the release of DNA to the extracellular milleu, in response to different stimuli. In this study, it was observed that the treatment of B and T cells with PMA, ionomycin and the serum from patients with SLE induced the extracellular DNA presence in B and T cells. These findings suggest that the phenomenon were similar to those observed in neutrophil's Etosis; B and T cells also released their DNA into the extracellular milieu. The findings express that serum from patients with SLE and SLEDAI ≤ 8 triggers the release of extracellular DNA in neutrophils, B and T cells, that suggested the presence of soluble factors in the serum that favoured this phenomenon.
在一种称为中性粒细胞胞外诱捕网形成（NETosis）的过程中，中性粒细胞将DNA释放到细胞外环境中，这一过程被认为是自身抗原的另一个来源；该过程被认为在某些自身免疫性疾病（如系统性红斑狼疮，SLE）的发病机制中起重要作用。然而，尚不清楚B细胞和T细胞是否会在不同刺激下将DNA释放到细胞外环境中。在本研究中，观察到用佛波酯（PMA）、离子霉素和SLE患者的血清处理B细胞和T细胞会导致B细胞和T细胞中出现细胞外DNA。这些发现表明，这一现象与在中性粒细胞胞外诱捕网形成中观察到的现象相似；B细胞和T细胞也将其DNA释放到细胞外环境中。研究结果表明，SLEDAI≤8的SLE患者血清会触发中性粒细胞、B细胞和T细胞释放细胞外DNA，这表明血清中存在促进这一现象的可溶性因子。
