Sathe Sharvari, Chan Xiang Quan, Jin Jing, Bernitt Erik, Döbereiner Hans-Günther, Yim Evelyn K F
Mechanobiology Institute, National University of Singapore, T-Lab, #05-01, 5A Engineering Drive 1, Singapore 117411.
Department of Biomedical Engineering, National University of Singapore, 4 Engineering Drive 3, Engineering Block 4, #04-08, Singapore 117583.
J Funct Biomater. 2017 Aug 12;8(3):35. doi: 10.3390/jfb8030035.
Neuronal morphology and differentiation have been extensively studied on topography. The differentiation potential of neural progenitors has been shown to be influenced by brain region, developmental stage, and time in culture. However, the neurogenecity and morphology of different neural progenitors in response to topography have not been quantitatively compared. In this study, the correlation between the morphology and differentiation of hippocampal and cortical neural progenitor cells was explored. The morphology of differentiated neural progenitors was quantified on an array of topographies. In spite of topographical contact guidance, cell morphology was observed to be under the influence of regional priming, even after differentiation. This influence of regional priming was further reflected in the correlations between the morphological properties and the differentiation efficiency of the cells. For example, neuronal differentiation efficiency of cortical neural progenitors showed a negative correlation with the number of neurites per neuron, but hippocampal neural progenitors showed a positive correlation. Correlations of morphological parameters and differentiation were further enhanced on gratings, which are known to promote neuronal differentiation. Thus, the neurogenecity and morphology of neural progenitors is highly responsive to certain topographies and is committed early on in development.
神经元形态与分化已在地形学方面得到广泛研究。神经祖细胞的分化潜能已被证明受脑区、发育阶段及培养时间的影响。然而，不同神经祖细胞对地形学的神经发生能力和形态尚未进行定量比较。在本研究中，探讨了海马体和皮质神经祖细胞形态与分化之间的相关性。在一系列地形学表面上对分化的神经祖细胞形态进行了量化。尽管存在地形接触导向，但即使在分化后，细胞形态仍受区域启动的影响。区域启动的这种影响在细胞形态特性与分化效率之间的相关性中进一步体现。例如，皮质神经祖细胞的神经元分化效率与每个神经元的神经突数量呈负相关，而海马体神经祖细胞则呈正相关。在已知能促进神经元分化的光栅上，形态参数与分化之间的相关性进一步增强。因此，神经祖细胞的神经发生能力和形态对某些地形学高度敏感，且在发育早期就已确定。
