Velthuys B R
Proc Natl Acad Sci U S A. 1979 Jun;76(6):2765-9. doi: 10.1073/pnas.76.6.2765.
With dark-adapted chloroplasts in which the plastoquinone was oxidized, a partial reduction of cytochrome b6 was obtained upon illumination with a pair of short saturating flashes. The second flash of the pair was much more effective than the first, and the reduction was inhibited by the system II inhibitor diuron. When the plastoquinone pool was reduced, both the reduction and the oxidation of cytochrome b6 were accelerated. The cytochrome b6 oxidation appeared to proceed in association with the reduction of cytochrome f, although these cytochromes are not simply connected in series. From these observations it is inferred that electron flow to the secondary donors of system I alternately caused the reduction and the oxidation of cytochrome b6. An interpretation is offered that also accounts for the transmembrane proton translocation that is associated with the oxidation of plastohydroquinone.
在质体醌被氧化的暗适应叶绿体中，用一对短的饱和闪光照射可使细胞色素b6部分还原。这一对闪光中的第二次闪光比第一次有效得多，并且这种还原被光系统II抑制剂敌草隆所抑制。当质体醌库被还原时，细胞色素b6的还原和氧化都加速。细胞色素b6的氧化似乎与细胞色素f的还原同时进行，尽管这些细胞色素并非简单地串联连接。从这些观察结果可以推断，向光系统I的二级供体的电子流交替地导致细胞色素b6的还原和氧化。本文还提供了一种解释，该解释也说明了与质体氢醌氧化相关的跨膜质子转运。
