Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam, University of Amsterdam, and MOVE Research Institute, VU University Amsterdam, Amsterdam, Netherlands.
CNRS INEE UMR 7262 - IPHEP, Institut de Paléoprimatologie et Paléontologie Humaine, Evolution et Paléoenvironnements, Université de Poitiers - Faculté des Sciences, Poitiers, Cedex 9, France.
Arch Oral Biol. 2017 Nov;83:214-221. doi: 10.1016/j.archoralbio.2017.07.018. Epub 2017 Jul 29.
Fluoride excess of 0.05-0.07mgF/kgbw/day in water or food additives like salt is the principal cause of endemic dental fluorosis. How fluoride causes these defects is not clear yet. Recent studies in rodents suggest that development of enamel fluorosis is associated with insufficient neutralization of protons released during the formation of hypermineralized lines.
Here we examined whether hypermineralization could also be assessed by MicroCT in developing molar enamel of humans exposed to fluoride.
Micro-CT analysis of hypomineralized enamel from human fluorotic molars graded by the Thylstrup-Fejerskov (TF) Index as III-IV showed weak hypermineralized lines and hypermineralized patches not seen in TF-I/II grade enamel. The mesio-distal sides of these molar teeth were significantly smaller (∼18%, p=0.02) than in TF-I/II teeth.
The patterns of changes observed in human fluorotic teeth were similar to those in fluorotic rodent incisors. The data are consistent with the hypothesis that also in developing human teeth fluoride-stimulated local acidification of enamel could be a mechanism for developing fluorotic enamel.
水或盐等食物添加剂中氟含量超过 0.05-0.07mgF/kgbw/day 是导致地方性氟斑牙的主要原因。然而，氟是如何导致这些缺陷的还不清楚。最近在啮齿动物中的研究表明，釉质氟斑的发生与在形成过度矿化线过程中释放的质子的中和不足有关。
在这里，我们研究了在暴露于氟化物的人类发育中的磨牙釉质中，是否也可以通过 MicroCT 来评估过度矿化。
通过 Micro-CT 分析，根据 Thylstrup-Fejerskov (TF) 指数分级为 III-IV 的氟斑牙釉质显示出较弱的过度矿化线和 TF-I/II 级釉质中未见的过度矿化斑块。这些磨牙的近远中面明显较小（约 18%，p=0.02）。
在人类氟斑牙中观察到的变化模式与氟斑鼠门牙中的相似。这些数据与假设一致，即在发育中的人类牙齿中，氟化物刺激的釉质局部酸化也可能是形成氟斑牙釉质的机制。
