Forsberg David, Thonabulsombat Charoensri, Jäderstad Johan, Jäderstad Linda Maria, Olivius Petri, Herlenius Eric
Department of Women's and Children's Health, Karolinska Institutet, and Karolinska University Hospital, Stockholm, Sweden.
Department of Clinical Sciences, Intervention and Technology (CLINTEC), Section of Otorhinolaryngology, Karolinska Institutet, and Karolinska University Hospital, Stockholm, Sweden.
Curr Protoc Stem Cell Biol. 2017 Aug 14;42:2D.13.1-2D.13.30. doi: 10.1002/cpsc.34.
Re-formation or preservation of functional, electrically active neural networks has been proffered as one of the goals of stem cell-mediated neural therapeutics. A primary issue for a cell therapy approach is the formation of functional contacts between the implanted cells and the host tissue. Therefore, it is of fundamental interest to establish protocols that allow us to delineate a detailed time course of grafted stem cell survival, migration, differentiation, integration, and functional interaction with the host. One option for in vitro studies is to examine the integration of exogenous stem cells into an existing active neural network in ex vivo organotypic cultures. Organotypic cultures leave the structural integrity essentially intact while still allowing the microenvironment to be carefully controlled. This allows detailed studies over time of cellular responses and cell-cell interactions, which are not readily performed in vivo. This unit describes procedures for using organotypic slice cultures as ex vivo model systems for studying neural stem cell and embryonic stem cell engraftment and communication with CNS host tissue. © 2017 by John Wiley & Sons, Inc.
功能性、电活性神经网络的重新形成或保存已被视为干细胞介导的神经治疗的目标之一。细胞治疗方法的一个主要问题是植入细胞与宿主组织之间形成功能性连接。因此，建立能够让我们描绘移植干细胞存活、迁移、分化、整合以及与宿主功能性相互作用详细时间进程的方案具有根本重要性。体外研究的一种选择是在离体器官型培养物中检查外源性干细胞与现有活性神经网络的整合情况。器官型培养物基本保持结构完整性，同时仍允许对微环境进行仔细控制。这使得能够随着时间推移对细胞反应和细胞间相互作用进行详细研究，而这在体内不易进行。本单元描述了使用器官型切片培养物作为离体模型系统来研究神经干细胞和胚胎干细胞植入以及与中枢神经系统宿主组织通讯的程序。© 2017约翰威立父子出版公司。
