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雄激素受体在小鼠心肌细胞的产前和产后早期发育阶段表达。

Androgen receptor is expressed in mouse cardiomyocytes at prenatal and early postnatal developmental stages.

作者信息

Pedernera Enrique, Gómora María José, Meneses Iván, De Ita Marlon, Méndez Carmen

机构信息

Facultad de Medicina, Edificio E, Universidad Nacional Autónoma de México, Av. Universidad #3000, Coyoacán, 04510, Cd. de México, CP, Mexico.

出版信息

BMC Physiol. 2017 Aug 14;17(1):7. doi: 10.1186/s12899-017-0033-8.

DOI:10.1186/s12899-017-0033-8
PMID:28806941
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5557468/
Abstract

BACKGROUND

Previous studies show that androgens are involved in hypertrophy and excitability of cardiomyocytes and that their effects are mediated through their receptor. The aim of this study was to evaluate the presence of androgen receptor (AR) in mouse heart during prenatal and early postnatal stages.

RESULTS

The expression of AR and related genes, alpha myosin heavy chain -Myh6-, beta myosin heavy chain -Myh7- and atrial natriuretic factor -Nppa- was simultaneously evaluated by semiquantitative RT-PCR. AR was also detected by immunohistochemistry. Androgen receptor mRNA was detected in hearts from 10.5 days post coitum to 16 postnatal days. A higher expression of AR mRNA in atria compared to ventricles was observed in neonatal mouse. A positive correlation between mRNA levels of AR and Nppa was observed in mouse heart at early postnatal development. Androgen receptor expression is similar in males and females during cardiac development. Finally, androgen receptor protein was observed by immunohistochemistry in myocardial cells of atria and ventricles from 12.5 days onwards and restricted after 16.5 days post-coitum to nuclei of cardiomyocytes.

CONCLUSION

Present results provide evidence that androgen receptor is expressed from prenatal stages in mouse heart, supporting the proposition that androgens could be involved in mammalian heart development.

摘要

背景

先前的研究表明，雄激素参与心肌细胞的肥大和兴奋性调节，且其作用通过其受体介导。本研究旨在评估产前和产后早期小鼠心脏中雄激素受体（AR）的存在情况。

结果

通过半定量逆转录聚合酶链反应（RT-PCR）同时评估AR及相关基因α-肌球蛋白重链（Myh6）、β-肌球蛋白重链（Myh7）和心钠素（Nppa）的表达。还通过免疫组织化学检测AR。在交配后10.5天至出生后16天的心脏中检测到雄激素受体信使核糖核酸（mRNA）。在新生小鼠中，观察到心房中AR mRNA的表达高于心室。在出生后早期发育阶段，小鼠心脏中AR mRNA水平与Nppa之间存在正相关。在心脏发育过程中，雄性和雌性的雄激素受体表达相似。最后，通过免疫组织化学在交配后12.5天起的心房和心室心肌细胞中观察到雄激素受体蛋白，在交配后16.5天后局限于心肌细胞核。

结论

目前的结果提供了证据，表明雄激素受体在小鼠心脏的产前阶段就已表达，支持雄激素可能参与哺乳动物心脏发育的观点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a5f/5557468/163f58a06ad0/12899_2017_33_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a5f/5557468/bc9537812cc3/12899_2017_33_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a5f/5557468/278e26855969/12899_2017_33_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a5f/5557468/8b005b309440/12899_2017_33_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a5f/5557468/0417625e60f1/12899_2017_33_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a5f/5557468/163f58a06ad0/12899_2017_33_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a5f/5557468/bc9537812cc3/12899_2017_33_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a5f/5557468/278e26855969/12899_2017_33_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a5f/5557468/8b005b309440/12899_2017_33_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a5f/5557468/0417625e60f1/12899_2017_33_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7a5f/5557468/163f58a06ad0/12899_2017_33_Fig5_HTML.jpg

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