Zhang Y, Wang S, Zheng Y, Liu A
Department of Otolaryngology - Head and Neck Surgery,Tongji Hospital,Huazhong University of Science and Technology,Wuhan,China.
Department of Otolaryngology - Head and Neck Surgery,Central Hospital of Wuhan,Huazhong University of Science and Technology,Wuhan,China.
J Laryngol Otol. 2017 Oct;131(10):860-865. doi: 10.1017/S0022215117001566. Epub 2017 Aug 15.
This study aimed to investigate the expression of DKK1 protein in an experimental model of tympanosclerosis and its possible role in the pathogenesis of this disorder.
Forty Sprague Dawley rats were included in the study: 20 in the control group (which received no treatment) and 20 in the experimental group (which received an incision to induce tympanosclerosis). Otomicroscopy was performed to observe the development of myringosclerosis. Haematoxylin and eosin staining was performed to observe the morphological changes. Western blot analysis and immunohistochemistry were performed to assess the expression of DKK1 protein.
At day 15, sclerotic lesions were observed in 70 per cent of the tympanic membranes. Inflammatory infiltration and hyaline degeneration markedly appeared in the tympanic membranes and middle-ear mucosa. DKK1 protein was mainly distributed in the cytoplasm of epithelial cells, which were widely distributed in the tympanic membranes and middle-ear mucosa. The expression of DKK1 protein was significantly decreased in the calcified experimental ears.
DKK1 protein is involved in the pathogenesis of tympanosclerosis by regulating the Wnt/β-catenin signalling pathway.
本研究旨在探讨硬化性中耳炎实验模型中DKK1蛋白的表达及其在该疾病发病机制中的可能作用。
40只Sprague Dawley大鼠纳入本研究：20只作为对照组（未接受任何治疗），20只作为实验组（接受诱导硬化性中耳炎的切口手术）。进行耳显微镜检查以观察鼓膜硬化的发展情况。采用苏木精-伊红染色观察形态学变化。通过蛋白质免疫印迹分析和免疫组织化学评估DKK1蛋白的表达。
第15天时，70%的鼓膜出现硬化性病变。鼓膜和中耳黏膜明显出现炎症浸润和透明变性。DKK1蛋白主要分布于上皮细胞的细胞质中，上皮细胞广泛分布于鼓膜和中耳黏膜。钙化的实验耳中DKK1蛋白的表达显著降低。
DKK1蛋白通过调节Wnt/β-连环蛋白信号通路参与硬化性中耳炎的发病机制。
