Polat Senol, Oztürk Ozmen, Uneri Cüneyd, Yüksel Meral, Haklar Goncagül, Bozkurt Süheyla, Küllü Sevgi
Department of Otorhinolaryngology-Head and Neck Surgery, Marmara University School of Medicine, Istanbul, Turkey.
Laryngoscope. 2004 Apr;114(4):720-5. doi: 10.1097/00005537-200404000-00023.
OBJECTIVES/HYPOTHESIS: Recent studies have established a strong relationship between the development of myringosclerosis and reactive oxygen species (ROS). The aims of the present study were to directly detect ROS in the tympanic membrane and middle ear mucosa of rats by measuring luminol amplified chemiluminescence, to evaluate the changes in the levels of ROS after treatment with vitamin E, and to examine the possible changes in the tympanic membranes otomicroscopically and histologically.
Prospective controlled animal study.
Forty healthy Sprague-Dawley rats were divided into five groups of eight animals each. Animals in all groups except group 1 were bilaterally myringotomized. Group 2 received no treatment, group 3 was treated with topical olive oil, group 4 received topical vitamin E, and group 5 received intramuscular vitamin E. After 24 hours of myringotomy, tympanic membranes were examined otomicroscopically; thereafter, tympanic membranes and middle ear mucosa were peeled off. The right ears of the animals were used for biochemical assay, and the left ears were used for histological study.
Reactive oxygen species levels were significantly decreased in group 4 with topical application of vitamin E compared with untreated and myringotomized animals in group 2. Reactive oxygen species levels were also decreased in group 5, although the decrease was not statistically significant when compared with groups 2 and 3. Histological studies confirmed sclerotic changes in the untreated myringotomized animals. The tympanic membranes of animals in groups 2 and 3 showed a white, chalk-like pattern of sclerotic changes, whereas animals in groups 4 and 5, with the exception of two animals in group 5, lacked these changes.
Although the relationship between the development of myringosclerosis and ROS had been well documented previously, the present study is the first that has directly measured the levels of ROS in the tympanic membrane and middle ear mucosa. These results are relevant because they correlate with histological findings. It has also been demonstrated that topically applied vitamin E is effective in decreasing the ROS levels.
目的/假设：最近的研究已证实鼓膜硬化的发展与活性氧（ROS）之间存在密切关系。本研究的目的是通过测量鲁米诺增强化学发光直接检测大鼠鼓膜和中耳黏膜中的ROS，评估维生素E治疗后ROS水平的变化，并通过耳显微镜和组织学检查鼓膜可能发生的变化。
前瞻性对照动物研究。
40只健康的Sprague-Dawley大鼠被分为五组，每组8只。除第1组外，所有组的动物均进行双侧鼓膜切开术。第2组不接受治疗，第3组用局部橄榄油治疗，第4组接受局部维生素E治疗，第5组接受肌肉注射维生素E。鼓膜切开术后24小时，用耳显微镜检查鼓膜；此后，剥离鼓膜和中耳黏膜。动物的右耳用于生化分析，左耳用于组织学研究。
与未治疗和鼓膜切开术的第2组动物相比，局部应用维生素E的第4组活性氧水平显著降低。第5组的活性氧水平也有所降低，尽管与第2组和第3组相比，降低幅度无统计学意义。组织学研究证实未治疗的鼓膜切开术动物存在硬化改变。第2组和第3组动物的鼓膜呈现白色、粉笔样的硬化改变模式，而第4组和第5组动物（第5组除外两只动物）则无这些改变。
虽然此前已充分证明鼓膜硬化的发展与ROS之间的关系，但本研究是首次直接测量鼓膜和中耳黏膜中ROS的水平。这些结果具有相关性，因为它们与组织学结果相关联。还证明了局部应用维生素E可有效降低ROS水平。
