Department of Medical Biochemistry and Clinical Biochemistry, (Firat Hormones Research Group), Medical School, Firat University, 23119 Elazig, Turkey.
Department of General Surgery, Medical School, Dokuz Eylul University, 35340 İzmir, Turkey.
Life Sci. 2017 Oct 1;186:102-110. doi: 10.1016/j.lfs.2017.08.013. Epub 2017 Aug 12.
The purpose of our investigative work has been to determine whether there can be therapeutic roles in the administration of sildenafil citrate, heparin and several neuropeptides on an animal model where gastric ulcers were induced with acetic acid, and to compare their efficacy.
The animals were divided into 13 groups, with 4 animals in each. Gastric ulcers was induced in the animals of 12 groups with one untreated group being left as the control (Group I - control; given normal saline (NS)). The other groups were: Group II (ulcer+NS); Group III (5mg/kg sildenafil citrate, low dose); Group IV (10mg/kg sildenafil citrate, high dose); Group V (0.6mg/kg heparin, low dose); Group VI (6mg/kg heparin, high dose); Group VII (20nmol/kg des-acyl ghrelin); Group VIII (40nmol/kg des-acyl ghrelin); Group IX (4nmol/kg acyl ghrelin); Group X (8nmol/kg acly ghrelin); Group XI (20pmol/kg Nesfatin-1); Group XII (15nmol/kg Obestatin) and Group XIII (5nmol/kg Neuropeptide Y). Gastric neuropeptide expression was measured using an immunohistochemical method, and the amount in circulation was detected using ELISA. To compare with no treatment, the controls and other treatment groups, we recorded loss of the surface epithelium of the stomach, erosion, bleeding and inflammatory cell infiltration in the upper halves of the gastric glands.
The muscularis and the layers beneath it were, however, apparently normal. The gastric mucosa healed with little or no inflammation when sildenafil citrate, low dose heparin, ghrelin, NUCB2/Nesfatin-1, obestatin, Neuropeptide Y were administered.
Overall the data indicate that low dose heparin, and especially sildenafil citrate and neuropeptides, can be used clinically as an alternative approach in the treatment of the gastric ulcer.
我们的研究目的是确定在乙酸诱导的胃溃疡动物模型中，给予枸橼酸西地那非、肝素和几种神经肽是否具有治疗作用，并比较它们的疗效。
将动物分为 13 组，每组 4 只。12 组动物诱导胃溃疡，一组未处理动物作为对照（I 组-对照；给予生理盐水（NS））。其他组为：II 组（溃疡+NS）；III 组（5mg/kg 枸橼酸西地那非，低剂量）；IV 组（10mg/kg 枸橼酸西地那非，高剂量）；V 组（0.6mg/kg 肝素，低剂量）；VI 组（6mg/kg 肝素，高剂量）；VII 组（20nmol/kg 去酰基胃饥饿素）；VIII 组（40nmol/kg 去酰基胃饥饿素）；IX 组（4nmol/kg 酰基胃饥饿素）；X 组（8nmol/kg 酰基胃饥饿素）；XI 组（20pmol/kg Nesfatin-1）；XII 组（15nmol/kg Obestatin）和 XIII 组（5nmol/kg Neuropeptide Y）。采用免疫组织化学方法测量胃神经肽表达，采用 ELISA 检测循环中的含量。与未治疗组、对照组和其他治疗组相比，我们记录了胃表面上皮的丧失、侵蚀、出血和胃腺上半部分的炎症细胞浸润。
然而，肌层和其下方的层显然是正常的。当给予低剂量肝素、西地那非、胃饥饿素、Nucb2/Nesfatin-1、obestatin、神经肽 Y 时，胃黏膜愈合，炎症很少或没有。
总的来说，数据表明，低剂量肝素，尤其是西地那非和神经肽，可以作为治疗胃溃疡的临床替代方法。
