Takeuti Karine L, de Barcellos David E S N, Pieters Maria
Department of Animal Medicine, College of Veterinary Medicine, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil; Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, United States.
Department of Animal Medicine, College of Veterinary Medicine, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
J Microbiol Methods. 2017 Oct;141:118-120. doi: 10.1016/j.mimet.2017.08.007. Epub 2017 Aug 12.
The objective of this study was to compare the absorption and M. hyopneumoniae detection by real-time PCR using nylon-flocked and rayon-bud swabs. Results of this study showed that absorption and detection of M. hyopneumoniae were significantly higher in nylon-flocked swabs with Ct differences only ranging from 0.5 to 1.7.
本研究的目的是比较使用尼龙植绒拭子和人造纤维头拭子通过实时PCR检测猪肺炎支原体的吸收情况和检测结果。本研究结果表明，尼龙植绒拭子对猪肺炎支原体的吸收和检测显著更高，Ct值差异仅在0.5至1.7之间。
