Zhang Yi, Liang Yingbo, Dong Yijie, Gao Yuhan, Yang Xiufen, Yuan Jingjing, Qiu Dewen
The State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 12 Zhong-guan-cun South Street, Beijing 100081, China.
The State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 12 Zhong-guan-cun South Street, Beijing 100081, China.
Biochem Biophys Res Commun. 2017 Oct 7;492(1):55-60. doi: 10.1016/j.bbrc.2017.08.039. Epub 2017 Aug 12.
MoHrip1, a protein isolated from Magnaporthe oryzae, belongs to the Alt A 1 (AA1) family. mohrip1 mRNA levels showed inducible expression throughout the infection process in rice. To determine the location of MoHrip1 in M. oryzae, a mohrip1-gfp mutant was generated. Fluorescence microscopy observations and western blotting analysis showed that MoHrip1 was both present in the secretome and abundant in the fungal cell wall. To obtain MoHrip1 protein, we carried out high-yield expression of MoHrip1 in Pichia pastoris. Treatment of tobacco plants with MoHrip1 induced the formation of necrosis, accumulation of reactive oxygen species and expression of several defense-related genes, as well as conferred disease resistance. By fusion to green fluorescent protein, we showed that MoHrip1 was able to bind to the tobacco and rice plant plasma membrane, causing rapid morphological changes at the cellular level, such as cell shrinkage and chloroplast disorganization. These findings indicate that MoHrip1 is a microbe-associated molecular pattern that is perceived by the plant immune system. This is the first study on an AA1 family protein that can bind to the plant plasma membrane.
MoHrip1是一种从稻瘟病菌中分离出的蛋白质，属于Alt A 1（AA1）家族。MoHrip1的mRNA水平在水稻整个感染过程中呈现出诱导型表达。为了确定MoHrip1在稻瘟病菌中的定位，构建了一个mohrip1 - gfp突变体。荧光显微镜观察和蛋白质免疫印迹分析表明，MoHrip1既存在于分泌组中，也大量存在于真菌细胞壁中。为了获得MoHrip1蛋白，我们在毕赤酵母中进行了MoHrip1的高产表达。用MoHrip1处理烟草植株会诱导坏死的形成、活性氧的积累以及几个防御相关基因的表达，还能赋予抗病性。通过与绿色荧光蛋白融合，我们发现MoHrip1能够结合到烟草和水稻植株的质膜上，在细胞水平上引起快速的形态变化，如细胞收缩和叶绿体解体。这些发现表明，MoHrip1是一种能被植物免疫系统识别的微生物相关分子模式。这是关于一种能结合植物质膜的AA1家族蛋白的首次研究。
