The State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
PLoS One. 2017 Apr 18;12(4):e0175734. doi: 10.1371/journal.pone.0175734. eCollection 2017.
Exogenous application of the protein elicitors MoHrip1 and MoHrip2, which were isolated from the pathogenic fungus Magnaporthe oryzae (M. oryzae), was previously shown to induce a hypersensitive response in tobacco and to enhance resistance to rice blast. In this work, we successfully transformed rice with the mohrip1 and mohrip2 genes separately. The MoHrip1 and MoHrip2 transgenic rice plants displayed higher resistance to rice blast and stronger tolerance to drought stress than wild-type (WT) rice and the vector-control pCXUN rice. The expression of salicylic acid (SA)- and abscisic acid (ABA)-related genes was also increased, suggesting that these two elicitors may trigger SA signaling to protect the rice from damage during pathogen infection and regulate the ABA content to increase drought tolerance in transgenic rice. Trypan blue staining indicated that expressing MoHrip1 and MoHrip2 in rice plants inhibited hyphal growth of the rice blast fungus. Relative water content (RWC), water usage efficiency (WUE) and water loss rate (WLR) were measured to confirm the high capacity for water retention in transgenic rice. The MoHrip1 and MoHrip2 transgenic rice also exhibited enhanced agronomic traits such as increased plant height and tiller number.
先前的研究表明，从病原菌稻瘟病菌（Magnaporthe oryzae）中分离得到的蛋白激发子 MoHrip1 和 MoHrip2 可以诱导烟草产生过敏反应，并增强对稻瘟病的抗性。在本工作中，我们成功地分别将 mohrip1 和 mohrip2 基因转入水稻中。与野生型（WT）水稻和载体对照 pCXUN 水稻相比，MoHrip1 和 MoHrip2 转基因水稻对稻瘟病的抗性更高，对干旱胁迫的耐受性更强。水杨酸（SA）和脱落酸（ABA）相关基因的表达也增加了，这表明这两种激发子可能触发 SA 信号来保护水稻免受病原菌感染的损伤，并调节 ABA 含量以提高转基因水稻的耐旱性。台盼蓝染色表明，在水稻植株中表达 MoHrip1 和 MoHrip2 抑制了稻瘟病菌的菌丝生长。通过测量相对含水量（RWC）、水分利用效率（WUE）和水分损失率（WLR）来确认转基因水稻具有较强的保水能力。MoHrip1 和 MoHrip2 转基因水稻还表现出增强的农艺性状，如株高和分蘖数的增加。
