Deng Ting, Ge Haoran, He Huahua, Liu Yao, Zhai Chao, Feng Liang, Yi Li
Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei University, Wuhan, 430062, China.
School of Environmental Studies, China University of Geosciences, 430074, China.
Protein Expr Purif. 2017 Dec;140:52-59. doi: 10.1016/j.pep.2017.08.003. Epub 2017 Aug 12.
Antimicrobial peptides (AMPs) consist of molecules acting on the defense systems of numerous organisms toward tumor and multiple pathogens, such as bacteria, fungi, viruses, and parasites. Compared to traditional antibiotics, AMPs are more stable and have lower propensity for developing resistance through functioning in the innate immune system, thus having important applications in the fields of medicine, food and so on. However, despite of their high economic values, the low yield and the cumbersome extraction process in AMPs production are problems that limit their industrial application and scientific research. To conquer these obstacles, optimized heterologous expression technologies were developed that could provide effective ways to increase the yield of AMPs. In this review, the research progress on heterologous expression of AMPs using Escherichia coli, Bacillus subtilis, Pichia pastoris and Saccharomyces cerevisiae as host cells was mainly summarized, which might guide the expression strategies of AMPs in these cells.
抗菌肽（AMPs）是一类作用于多种生物体防御系统以对抗肿瘤和多种病原体（如细菌、真菌、病毒和寄生虫）的分子。与传统抗生素相比，抗菌肽更稳定，且通过在先天免疫系统中发挥作用产生耐药性的倾向较低，因此在医学、食品等领域具有重要应用。然而，尽管抗菌肽具有很高的经济价值，但其产量低以及生产过程中提取工艺繁琐，这些问题限制了它们在工业应用和科学研究中的发展。为克服这些障碍，人们开发了优化的异源表达技术，可为提高抗菌肽产量提供有效途径。在本综述中，主要总结了以大肠杆菌、枯草芽孢杆菌、毕赤酵母和酿酒酵母作为宿主细胞异源表达抗菌肽的研究进展，这可能会指导抗菌肽在这些细胞中的表达策略。
