Mosallayi Meysam, Simonian Miganoosh, Khosravi Sharifeh, Salehi Ahmad Reza, Khodadoostan Mahsa, Sebghatollahi Vahid, Baradaran Azar, Salehi Rasoul
Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Internal Medicine, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Adv Biomed Res. 2017 Jul 14;6:77. doi: 10.4103/2277-9175.210656. eCollection 2017.
One of the gene expression regulatory mechanisms is mediated by small noncoding RNAs called microRNA (miRNA). They interact with a recognition sequence located mostly in 3'-untranslated regions (3'-UTRs) of mRNAs. Polymorphisms in miRNAs recognition sequences could affect gene expression which in turn may alter disease susceptibility. , a member of the domain-containing methyltransferase, acts in a variety of biological processes such as genomic stability. Here, we report correlation of rs16917496 polymorphism, located in the recognition sequence of miR-502 within 3'-UTR of , with colorectal cancer (CRC) in Iranians.
One hundred and seventy CRC patients and 170 noncancer counterparts were recruited in this case-control study. Genotyping of rs16917496 was performed using polymerase chain reaction-restriction fragment length polymorphism method.
There was no significant association of rs16917496 with CRC in population under study ( value for genotype and allele distribution were >0.05). However, stratification analysis based on smoking status revealed that TT+TC genotypes of rs16917496 are strongly associated with increased risk of CRC (odds ratio: 5.8, 95% confidence interval: 1.37-24.34, - 0.005) in smoker subgroup.
Correlation of rs16917496 T allele with CRC in smokers is emphasizing the importance of individuals' genotype in the recruitment of adverse health hazards of smoking more profoundly for certain people compared to others.
基因表达调控机制之一是由称为微小RNA（miRNA）的小非编码RNA介导的。它们与主要位于mRNA的3'非翻译区（3'-UTR）的识别序列相互作用。miRNA识别序列中的多态性可能影响基因表达，进而可能改变疾病易感性。含 结构域的甲基转移酶成员 在多种生物学过程如基因组稳定性中发挥作用。在此，我们报告了位于 的3'-UTR内miR-502识别序列中的rs16917496多态性与伊朗人大肠癌（CRC）的相关性。
在这项病例对照研究中招募了170例CRC患者和170例非癌症对照者。使用聚合酶链反应-限制性片段长度多态性方法对rs16917496进行基因分型。
在研究人群中，rs16917496与CRC无显著关联（基因型和等位基因分布的 值均>0.05）。然而，基于吸烟状况的分层分析显示，在吸烟者亚组中，rs16917496的TT + TC基因型与CRC风险增加密切相关（优势比：5.8，95%置信区间：1.37 - 24.34，P = 0.005）。
rs16917496 T等位基因与吸烟者CRC的相关性更深刻地强调了个体基因型在某些人比其他人更容易受到吸烟不良健康危害方面的重要性。
