贝宁 1 型糖尿病与 HLA Ⅱ类等位基因、单倍型和基因型的相关性：一项初步研究。
HLA Class II Allele, Haplotype, and Genotype Associations with Type 1 Diabetes in Benin: A Pilot Study.
机构信息
UFR de Biologie Humaine, Faculté des Sciences de la Santé, Université d'Abomey-Calavi, Cotonou, Benin.
Laboratoire de Recherche en Biologie Appliquée, Ecole Polytechnique d'Abomey-Calavi, Université d'Abomey-Calavi, Abomey-Calavi, Benin.
出版信息
J Diabetes Res. 2017;2017:6053764. doi: 10.1155/2017/6053764. Epub 2017 Jul 20.
BACKGROUND
Several studies have reported the implication of HLA-DR/DQ loci in the susceptibility to type 1 diabetes (T1D). Since no such study has yet been performed in Benin, this pilot one aimed at assessing HLA class II allele, haplotype, and genotype associations with T1D.
MATERIAL AND METHODS
Class II HLA genotyping was performed in 51 patients with T1D and 51 healthy unrelated controls by means of the PCR-SSP method. The diagnosis of T1D was set up according to American Diabetes Association criteria. Odds ratio (OR) and its 95% confidence interval (95% CI) were calculated to assess the associations between T1D and HLA alleles, haplotypes, and genotypes.
RESULTS
Participants were aged 1-24 years. T1D was significantly associated with DR3, DQA1∗05:01, DQB1∗02:01, and DR3-DR4. No significant associations were observed with DR4, DQB1∗03:02, and DQB1∗06:02.
CONCLUSION
Certain HLA class II alleles, haplotypes, and genotypes were related to T1D and may be used as genetic susceptibility markers to T1D in Benin.
背景
几项研究报告了 HLA-DR/DQ 基因座在 1 型糖尿病（T1D）易感性中的作用。由于贝宁尚未进行此类研究，因此本研究旨在评估 HLA Ⅱ类等位基因、单倍型和基因型与 T1D 的关联。
材料与方法
采用 PCR-SSP 法对 51 例 T1D 患者和 51 例无关健康对照进行Ⅱ类 HLA 基因分型。根据美国糖尿病协会标准诊断 T1D。计算比值比（OR）及其 95%置信区间（95%CI），以评估 T1D 与 HLA 等位基因、单倍型和基因型之间的关联。
结果
参与者年龄为 1-24 岁。T1D 与 DR3、DQA1∗05:01、DQB1∗02:01 和 DR3-DR4 显著相关。与 DR4、DQB1∗03:02 和 DQB1∗06:02 无显著关联。
结论
某些 HLA Ⅱ类等位基因、单倍型和基因型与 T1D 相关，可能作为贝宁 T1D 的遗传易感性标志物。
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