Gui Ee Mei, Lu Ting, Teo Tang Lin, Cheow Pui Sze, Lee Tong Kooi
Chemical Metrology Laboratory, Chemical Metrology Division, Applied Sciences Group, Health Sciences Authority, 1 Science Park Road, #01-05/06, The Capricorn, Singapore Science Park II, Singapore, 117528, Singapore.
Anal Bioanal Chem. 2017 Oct;409(26):6069-6080. doi: 10.1007/s00216-017-0544-y. Epub 2017 Aug 14.
A gas chromatography-isotope dilution mass spectrometry (GC-IDMS) technique was developed for the quantification of two heavy polyaromatic hydrocarbons (PAHs), benz[a]anthracene and benzo[a]pyrene, in yerba maté tea (maté). The optimisation of two extraction methods, namely liquid-liquid extraction and accelerated solvent extraction, was carried out. Both optimised methods were validated using a certified reference material of fine dust and the results were within the expanded uncertainties at 95% confidence level. Recoveries of 99.2-106.7% with RSD of measurements of 1.1-2.3% were achieved for benz[a]anthracene. Recoveries of 95.7-101.9% with RSD of measurements of 0.4-1.4% were achieved for benzo[a]pyrene. The validated methods were applied for the extraction of benz[a]anthracene and benzo[a]pyrene in maté powder from NIST. A metrological approach was undertaken to ensure the traceability of measurement results. The uncertainties associated with the results were rigorously evaluated and also reported herein. Graphical abstract Quantification of benz[a]anthracene and benzo[a]pyrene using IDMS.
开发了一种气相色谱-同位素稀释质谱法（GC-IDMS），用于定量分析马黛茶（玛黛茶）中的两种重质多环芳烃（PAHs），即苯并[a]蒽和苯并[a]芘。对液液萃取和加速溶剂萃取这两种萃取方法进行了优化。两种优化方法均使用细粉尘标准参考物质进行了验证，结果在95%置信水平下的扩展不确定度范围内。苯并[a]蒽的回收率为99.2-106.7%，测量相对标准偏差（RSD）为1.1-2.3%。苯并[a]芘的回收率为95.7-101.9%，测量RSD为0.4-1.4%。经验证的方法用于从美国国家标准与技术研究院（NIST）提取马黛茶粉末中的苯并[a]蒽和苯并[a]芘。采用了一种计量方法来确保测量结果的可追溯性。对与结果相关的不确定度进行了严格评估，并在此报告。图形摘要 使用IDMS对苯并[a]蒽和苯并[a]芘进行定量分析。
