Musashi-1第二个RNA结合结构域在其游离形式以及与5聚体RNA形成复合物时的主链和侧链归属。
Backbone and side chain assignments of the second RNA-binding domain of Musashi-1 in its free form and in complex with 5-mer RNA.
作者信息
Iwaoka Ryo, Nagata Takashi, Tsuda Kengo, Imai Takao, Okano Hideyuki, Kobayashi Naohiro, Katahira Masato
机构信息
Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto, 611-0011, Japan.
Graduate School of Energy Science, Kyoto University, Gokasho, Uji, Kyoto, 611-0011, Japan.
出版信息
Biomol NMR Assign. 2017 Oct;11(2):265-268. doi: 10.1007/s12104-017-9760-9. Epub 2017 Aug 14.
Musashi1 (Msi1) is an RNA-binding protein that is involved in cell fate determination. Here, we report the H, N, and C resonance assignments of Msi1 second RNA-binding domain in free form and in complex with RNA. The assignments can be utilized for NMR structure and dynamics analyses of the Msi1:RNA complex, and moreover, for chemical shift perturbation analyses to evaluate the binding of potential small molecule inhibitors against Msi1:RNA interaction.
Musashi1（Msi1）是一种参与细胞命运决定的RNA结合蛋白。在此，我们报告了Msi1第二个RNA结合结构域在游离形式以及与RNA形成复合物时的H、N和C共振归属。这些归属可用于Msi1:RNA复合物的核磁共振结构和动力学分析，此外，还可用于化学位移扰动分析，以评估潜在小分子抑制剂对Msi1:RNA相互作用的结合情况。
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