Katzenell Sarah, Cabrera Jorge R, North Brian J, Leib David A
Department of Microbiology and Immunology, Geisel School of Medicine, Dartmouth University, 630E Borwell Building, One Medical Center Drive, HB 7556, Lebanon, NH, 03756, USA.
Methods Mol Biol. 2017;1656:229-251. doi: 10.1007/978-1-4939-7237-1_15.
Cultured primary neurons have been of extraordinary value for the study of neuronal anatomy, cell biology, and physiology. While use of neuronal cell lines has ease and utility, there are often caveats that arise due to their mitotic nature. This methods article presents detailed methodology for the preparation, purification, and culture of adult murine sensory neurons for the study of herpes simplex virus lytic and latent infections. While virology is the application for our laboratory, these cultures also have broad utility for neurobiologists and cell biologists. While these primary cultures have been highly informative, the methodology is challenging to many investigators. Through publication of this highly detailed protocol, it is our hope that the use of this culture system can spread in the field to allow more rapid progress in furthering our understanding of neurotropic virus infection.
原代培养的神经元对于神经元解剖学、细胞生物学和生理学的研究具有非凡的价值。虽然使用神经元细胞系操作简便且实用，但由于其有丝分裂的特性，往往存在一些问题。这篇方法学文章介绍了用于研究单纯疱疹病毒裂解性和潜伏性感染的成年小鼠感觉神经元的制备、纯化和培养的详细方法。虽然病毒学是我们实验室的应用领域，但这些培养物对神经生物学家和细胞生物学家也有广泛的用途。虽然这些原代培养物提供了丰富的信息，但该方法对许多研究者来说具有挑战性。通过发表这个高度详细的方案，我们希望这种培养系统能够在该领域得到更广泛的应用，以便在加深我们对嗜神经病毒感染的理解方面取得更快的进展。
