Macleod Kenneth G, Serrels Bryan, Carragher Neil O
Cancer Research UK Edinburgh Centre, Institute of Genetics and Molecular Medicine, University of Edinburgh, Crewe Road South, Edinburgh, EH4 2XR, UK.
Methods Mol Biol. 2017;1647:153-169. doi: 10.1007/978-1-4939-7201-2_10.
Reverse Phase Protein Arrays (RPPA) represent a sensitive antibody-based proteomic approach, which enables simultaneous quantification of the abundance of multiple proteins and posttranslational modifications across multiple samples. Here, we provide protocols for RPPA performed on two distinct protein-binding substrates associated with two most commonly used RPPA platform technologies. We compare and contrast the respective advantages and limitations of each platform within the context of drug discovery applications.
反向蛋白质阵列（RPPA）是一种基于抗体的灵敏蛋白质组学方法，能够同时对多个样品中的多种蛋白质丰度和翻译后修饰进行定量分析。在此，我们提供了在与两种最常用的RPPA平台技术相关的两种不同蛋白质结合底物上进行RPPA的实验方案。我们在药物发现应用的背景下比较并对比了每个平台各自的优缺点。
