Rummun N, Hughes R E, Beesoo R, Li W W, Aldulaimi O, Macleod K G, Bahorun T, Carragher N O, Kagansky A, Neergheen-Bhujun V S
Department of Health Sciences, Faculty of Science, University of Mauritius, Réduit, 80837, Republic of Mauritius.
ANDI Centre of Excellence for Biomedical and Biomaterials Research, MSIRI Building, University of Mauritius, Réduit, 80837, Republic of Mauritius.
Acta Naturae. 2019 Jan-Mar;11(1):81-90.
Terrestrial plants have contributed massively to the development of modern oncologic drugs. Despite the wide acceptance of Mauritian endemic flowering plants in traditional medicine, scientific evidence of their chemotherapeutic potential is lacking. This study aimed to evaluate the tumor cytotoxicity of leaf extracts from five Mauritian endemic medicinal plants, namely Willd (Euphorbiaceae), Bojer (Sapotaceae), Cav. subsp. Friedmann (Malvaceae), (DC.) Baker (Rubiaceae), and Lam (Myrtaceae). The cytotoxicities of the extracts were determined against six human cancer cell lines, including cervical adenocarcinoma, colorectal carcinoma, oesophageal adenocarcinoma, and oesophageal squamous cell carcinoma. The potent extracts were further investigated using cell cycle analysis and reverse phase protein array (RPPA) analysis. The antioxidant properties and polyphenolic profile of the potent extracts were also evaluated. Gas chromatography mass spectrometry (GC-MS) analyses revealed the presence of (+)-catechin and gallocatechin in and , while gallic acid was detected in . , and were highly selective towards human oesophageal squamous cell carcinoma (KYSE-30) cells. and arrested KYSE- 30 cells in the G2/M phase, in a concentration-dependent manner. RPPA analysis indicated that the extracts may partly exert their tumor growth-inhibitory activity by upregulating the intracellular level of 5'AMP-activated kinase (AMPK). The findings highlight the potent antiproliferative activity of three Mauritian endemic leaf extracts against oesophageal squamous cell carcinoma and calls for further investigation into their chemotherapeutic application.
陆生植物对现代肿瘤药物的发展做出了巨大贡献。尽管毛里求斯特有的开花植物在传统医学中被广泛接受，但其化疗潜力的科学证据仍然缺乏。本研究旨在评估五种毛里求斯特有的药用植物叶提取物的肿瘤细胞毒性，这五种植物分别是大戟科的Willd、山榄科的Bojer、锦葵科的Cav. subsp. Friedmann、茜草科的(DC.) Baker和桃金娘科的Lam。测定了这些提取物对六种人类癌细胞系的细胞毒性，包括宫颈腺癌、结肠直肠癌、食管腺癌和食管鳞状细胞癌。对活性提取物进一步进行细胞周期分析和反相蛋白质阵列（RPPA）分析。还评估了活性提取物的抗氧化性能和多酚谱。气相色谱 - 质谱（GC - MS）分析显示，在[具体植物1]和[具体植物2]中存在（+）-儿茶素和没食子儿茶素，而在[具体植物3]中检测到没食子酸。[具体植物1]、[具体植物2]和[具体植物3]对人类食管鳞状细胞癌（KYSE - 30）细胞具有高度选择性。[具体植物1]和[具体植物2]以浓度依赖的方式使KYSE - 30细胞停滞在G2/M期。RPPA分析表明，这些提取物可能通过上调细胞内5'AMP激活蛋白激酶（AMPK）的水平部分发挥其肿瘤生长抑制活性。这些发现突出了三种毛里求斯特有叶提取物对食管鳞状细胞癌的强大抗增殖活性，并呼吁进一步研究它们在化疗中的应用。
