Kuwayama Kenji, Miyaguchi Hajime, Iwata Yuko T, Kanamori Tatsuyuki, Tsujikawa Kenji, Yamamuro Tadashi, Segawa Hiroki, Inoue Hiroyuki
National Research Institute of Police Science, Chiba, Japan.
Drug Test Anal. 2018 Apr;10(4):750-760. doi: 10.1002/dta.2259. Epub 2017 Oct 17.
Segmental hair analysis is used to estimate the time of drug intake at monthly precision in drug-related crimes. Previously, we advanced this analytical method to specify the day of drug intake by cutting a strand of hair into 0.4-mm segments, which correspond to daily hair growth. Herein, we investigated the distributions of 7 compounds in a strand of hair using micro-segmental analysis. Several strands of hair were collected 33.1-229.4 days after subjects were administered 4 pharmaceutical products that contained 10 drugs in single doses within 32 hours. The administered drugs and resulting metabolites were extracted from 0.4-mm hair segments and quantified using liquid chromatography-tandem mass spectrometry. Acidic and neutral compounds were detected at low amounts in any of the hair segments analyzed. Epinastine, fexofenadine, dihydrocodeine, chlorpheniramine, and the chlorpheniramine metabolite, desmethylchlorpheniramine each was localized to 2 regions within a strand of hair. By contrast, methylephedrine and its metabolite, ephedrine, each was localized to only a region. Among 20 individual strands of hair associated with different subjects and head regions, few differences in the shapes of drug concentration-hair segment curves for each compound were detected. Our data indicated that 2 mechanisms for drug uptake into hair can operate depending on drug properties and that co-administered drugs can be localized to different regions in a strand of hair. Micro-segmental analysis may aid in the identification of the day of drug intake and help to elucidate the mechanisms of drug uptake into hair.
在与毒品相关的犯罪中，节段性毛发分析用于以每月的精度估计药物摄入时间。此前，我们改进了这种分析方法，通过将一缕头发剪成0.4毫米的片段（对应于每日头发生长）来确定药物摄入的日期。在此，我们使用微节段分析研究了一缕头发中7种化合物的分布。在受试者在32小时内单次服用含10种药物的4种药品后33.1 - 229.4天，收集了几缕头发。从0.4毫米的头发片段中提取给药药物及其产生的代谢物，并使用液相色谱 - 串联质谱法进行定量。在分析的任何头发片段中，酸性和中性化合物的含量都很低。依匹斯汀、非索非那定、二氢可待因、氯苯那敏以及氯苯那敏代谢物去甲基氯苯那敏各自都定位于一缕头发内的2个区域。相比之下，甲基麻黄碱及其代谢物麻黄碱各自仅定位于一个区域。在与不同受试者和头部区域相关的20缕单根头发中，未检测到每种化合物的药物浓度 - 头发片段曲线形状有明显差异。我们的数据表明，根据药物特性，药物进入头发的机制有两种，并且共同给药的药物可以定位于一缕头发中的不同区域。微节段分析可能有助于确定药物摄入的日期，并有助于阐明药物进入头发的机制。
