Stauss H J, Linsk R, Fischer A, Watts S, Banasiak D, Haberman A, Clark I, Forman J, McMillan M, Schreiber H
J Immunogenet. 1986 Apr-Jun;13(2-3):101-11. doi: 10.1111/j.1744-313x.1986.tb01090.x.
The C3H UV-induced fibrosarcoma, 1591, is highly immunogenic and, therefore, is readily rejected when transplanted into immunocompetent syngeneic recipients. Previous analysis of 1591 with tumour-specific or H-2-reactive monoclonal antibodies revealed that this antigenicity might be due to the expression of two novel class I major histocompatibility complex (MHC) antigens. In this report we describe the molecular cloning and initial characterization of three genes which account for all of the unique serological class I reactivities observed on this tumour. These include two distinct, but highly conserved, H-2L-like genes, and a third gene the product of which bears determinants which are characteristic of both the tumour and of class I products of the H-2k haplotype. Moreover, each of these genes contains a polymorphic restriction enzyme fragment which is detected in the class I sequences of 1591 relative to normal C3H tissue. Since the expression of these polymorphic class I sequences is relevant to the immunogenicity of 1591, the mutational events by which these genes were generated may be significant to the immunobiology of this tumour.
C3H紫外线诱导的纤维肉瘤1591具有高度免疫原性，因此，当移植到具有免疫活性的同基因受体中时很容易被排斥。先前用肿瘤特异性或H-2反应性单克隆抗体对1591进行分析发现，这种抗原性可能归因于两种新型I类主要组织相容性复合体（MHC）抗原的表达。在本报告中，我们描述了三个基因的分子克隆和初步表征，这三个基因解释了在该肿瘤上观察到的所有独特的血清学I类反应性。其中包括两个不同但高度保守的H-2L样基因，以及第三个基因，其产物带有肿瘤和H-2k单倍型I类产物特有的决定簇。此外，这些基因中的每一个都包含一个多态性限制性酶切片段，相对于正常C3H组织，该片段在1591的I类序列中被检测到。由于这些多态性I类序列的表达与1591的免疫原性相关，这些基因产生的突变事件可能对该肿瘤的免疫生物学具有重要意义。
