Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China.
Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin, China.
J Pharm Pharmacol. 2017 Nov;69(11):1540-1551. doi: 10.1111/jphp.12791. Epub 2017 Aug 15.
The purpose of this work was to determine and investigate the absorption of ginkgo terpenoids (GT) in plasma and aqueous humour after oral administration of ginkgo biloba extract (GBE) by UPLC-MS/MS method.
The UPLC-MS/MS determination of GT employed the multiple reaction monitoring mode using an electrospray negative ionization. The rabbits were orally administered the suspension of GBE at a dose of 500 mg/kg. Serial plasma and dialysate samples were collected at the corresponding time and then analysed by UPLC-MS/MS.
In plasma, the mean AUC from 0 to 48 h was 14.12, 12.59, 23.75, 1.51 h μg/ml for GLJ and 5.34 h μg/ml for GLA, GLB, GLC, GLJ and BLL, respectively. In aqueous humour, the five ginkgo terpenoids have been detected. Compared with the other four GT, BLL has better absorption in the eyes.
A selective and reproducible UPLC-MS/MS method was developed and validated to determine and investigate the absorption of ginkgo terpenoids in plasma and aqueous humour of rabbits after oral administration of GBE. The main five ginkgo terpenoids could be absorbed into eyes.
本工作旨在通过 UPLC-MS/MS 法测定并研究银杏叶提取物（GBE）口服后银杏萜类（GT）在血浆和房水中的吸收情况。
采用电喷雾负离子多反应监测模式的 UPLC-MS/MS 法测定 GT。兔子以 500mg/kg 的剂量口服给予 GBE 混悬液。在相应时间采集系列血浆和透析液样本，并用 UPLC-MS/MS 进行分析。
在血浆中，GLJ 的 AUC0-48h 平均值分别为 14.12、12.59、23.75、1.51hμg/ml，GLA、GLB、GLC、GLJ 和 BLL 的 AUC0-48h 平均值分别为 5.34hμg/ml。在房水中，检测到五种银杏萜类化合物。与其他四种 GT 相比，BLL 在眼部的吸收更好。
建立并验证了一种选择性和重现性好的 UPLC-MS/MS 法，用于测定并研究 GBE 口服后兔血浆和房水中银杏萜类的吸收情况。主要的五种银杏萜类化合物可被吸收到眼部。
