Karimi Fatemeh, Collins Joe, Heath Daniel E, Connal Luke A
School of Chemical and Biomedical Engineering, Particulate Fluids Processing Centre and ‡Polymer Science Group, Department of Chemical and Biomolecular Engineering, Particulate Fluids Processing Centre, University of Melbourne , Parkville, Melbourne, Victoria 3010, Australia.
Bioconjug Chem. 2017 Sep 20;28(9):2235-2240. doi: 10.1021/acs.bioconjchem.7b00360. Epub 2017 Aug 22.
A dual-responsive, cell capture and release surface was prepared through the incorporation of phenylboronic acid (PBA) groups into an oxime-based polyethylene glycol (PEG) hydrogel. Owing to its PEG-like properties, the unfunctionalized hydrogel was nonfouling. The use of highly efficient oxime chemistry allows the incorporation of commercially available 3,5-diformylphenyl boronic acid into the hydrogel matrix. Thus, the surface properties of the hydrogel were modified to enable reversible cell capture and release. Boronic ester formation between PBA groups and cell surface carbohydrates enabled efficient cell capture at pH 6.8. An increase to pH 7.8 resulted in cell detachment. This capture-and-release procedure was performed on MCF-7 human breast cancer cells, NIH-3T3 fibroblast cells, and primary human umbilical vein endothelial cells (HUVECs) and could be cycled with negligible loss in activity. The facile preparation of PBA-functionalized surfaces presented here has applications in biomedical fields such as cell diagnostics and cell culture.
通过将苯硼酸（PBA）基团引入肟基聚乙二醇（PEG）水凝胶中，制备了一种具有双重响应性的细胞捕获与释放表面。由于其类似PEG的性质，未功能化的水凝胶具有抗污性。高效肟化学的应用使得可将市售的3,5-二甲酰基苯硼酸引入水凝胶基质中。因此，水凝胶的表面性质得以改性，以实现细胞的可逆捕获与释放。PBA基团与细胞表面碳水化合物之间形成硼酸酯，使得在pH 6.8时能够高效捕获细胞。将pH提高到7.8会导致细胞 detachment。这种捕获-释放过程在MCF-7人乳腺癌细胞、NIH-3T3成纤维细胞和原代人脐静脉内皮细胞（HUVECs）上进行，并且可以循环进行，活性损失可忽略不计。本文介绍的PBA功能化表面的简便制备方法在细胞诊断和细胞培养等生物医学领域具有应用价值。
