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低温启动堆肥过程中接种冷适应微生物菌剂对酶活性的影响。

Effect of cold-adapted microbial agent inoculation on enzyme activities during composting start-up at low temperature.

机构信息

College of Life Science, Northeast Agricultural University, Harbin 150030, China.

College of Life Science, Northeast Agricultural University, Harbin 150030, China.

出版信息

Bioresour Technol. 2017 Nov;244(Pt 1):635-640. doi: 10.1016/j.biortech.2017.08.010. Epub 2017 Aug 5.

Abstract

In order to put forward a method to promote composting start-up at low ambient temperature, the cold-adapted microbial agent (CAMA) was inoculated in chicken manure (CM), and compared the enzymes activities, including urease, proteases, β-glucosidase and invertase, with no CAMA group (CK). In this study, the temperature of CM reached 50°C in 53h, but it in CK was only around 30°C during the composting process. Moreover, the enzymes exhibited higher activity in CM than CK, indicating the effectiveness of CAMA. Furthermore, redundancy analysis was conducted to study the relationships of CAMA, with enzymes activities and temperature. Results showed that the positive effect of CAMA on the enzyme activities were achieved by affecting the bacterial community structure. Accordingly, we provide a method to guide CAMA inoculation for promoting compost start-up in cold area.

摘要

为了提出一种促进低温环境下堆肥启动的方法,在鸡粪(CM)中接种了耐冷微生物菌剂(CAMA),并与未添加 CAMA 的对照组(CK)比较了脲酶、蛋白酶、β-葡萄糖苷酶和转化酶等酶活性。在这项研究中,CM 的温度在 53 小时内达到 50°C,而 CK 仅在堆肥过程中达到约 30°C。此外,CM 中的酶活性高于 CK,表明 CAMA 是有效的。此外,还进行了冗余分析以研究 CAMA 与酶活性和温度之间的关系。结果表明,CAMA 通过影响细菌群落结构对酶活性产生了积极影响。因此,我们提供了一种指导 CAMA 接种以促进寒冷地区堆肥启动的方法。

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