Zhang Jingtao, Li Chao, Xia Kai, Cheng Kaikai, Guo Shanhu, Pan Wenzheng, Liu Lingling, Wang Ke, Wen Li, Tang Haiming
China Tobacco Hunan Industrial Co., Ltd., Changsha, China.
Hunan Cultivated Land and Agricultural Eco-Environment Institute, Changsha, China.
Front Microbiol. 2025 Jul 22;16:1615412. doi: 10.3389/fmicb.2025.1615412. eCollection 2025.
Agent addition is increasingly recognized as a crucial strategy for improving soil health in tobacco cultivation. However, its impacts on soil microbial community and plant growth differ depending on soil conditions. In this study, tobacco soil was collected in Wu Ding County, Yunnan Province, under four distinct agent addition treatments, simply chemical fertilizer (T1), microbial agent + chemical fertilizer (T2), lime + chemical fertilizer (T3), and microbial agent + lime + chemical fertilizer (T4). Using the Illumina high-throughput sequencing platform and fungal ribosomal DNA internal transcribed spacer 1 to analyze the distribution characteristics of fungal communities in tobacco soil. Soil nutrient indicators (pH, SOC, TN and AP) were considerably higher in T4 than in T1. In comparison to T1, agent addition boosted the agronomic characteristics such as maximum leaf length, maximum leaf width, stem girth, and leaf number. T4 treatment facilitated the harmonization of chemical composition of tobacco leaves and greatly increased tobacco yield by 8.94% than T1. The application of T4 resulted in a reduction of fungal diversity. was the most dominant phylum across all soil and agent applications distinctly shifting the soil fungal community diversity. Furthermore, certain beneficial fungi were obviously accumulated, but the potentially pathogenic fungi were noticeably reduced or absent in T4. An explicit enrichment of saprotrophic fungi in T4 is predicated by the FUNGuild function. Soil nutrients were extremely significant and relevant in relation to the fungal community structure. In summary, we propose that T4 treatment could be an effective strategy to alleviate the continuous cropping barrier in tobacco cultivation since it eliminates soil acidity, improves soil nutrients, and modifies the soil microbial community structure, thereby improving the plant growth and increasing the yield of tobacco.
添加改良剂日益被认为是改善烟草种植土壤健康的关键策略。然而，其对土壤微生物群落和植物生长的影响因土壤条件而异。本研究在云南省武定县采集烟草种植土壤，设置了四种不同的改良剂添加处理，即单施化肥（T1）、微生物菌剂+化肥（T2）、石灰+化肥（T3）以及微生物菌剂+石灰+化肥（T4）。利用Illumina高通量测序平台和真菌核糖体DNA内转录间隔区1来分析烟草土壤中真菌群落的分布特征。T4处理下的土壤养分指标（pH、有机碳、全氮和有效磷）显著高于T1。与T1相比，添加改良剂提高了最大叶长、最大叶宽、茎围和叶片数等农艺性状。T4处理促进了烟叶化学成分的协调，烟叶产量比T1显著提高了8.94%。T4处理导致真菌多样性降低。在所有土壤和改良剂处理中， 是最主要的门，明显改变了土壤真菌群落多样性。此外，T4中某些有益真菌明显积累，而潜在致病真菌明显减少或不存在。基于FUNGuild功能预测，T4中腐生真菌有明显富集。土壤养分与真菌群落结构极显著相关。总之，我们认为T4处理可能是缓解烟草连作障碍的有效策略，因为它消除了土壤酸度，改善了土壤养分，改变了土壤微生物群落结构，从而促进了植物生长并提高了烟草产量。
