Xie Zonglan, Dong Ziqiang, Zhu Ping, Zhang Lusheng, Chen Xiaobo, Dong Chuanjiang
The Department of Urology, The First College of Clinical Medical Science, China Three Gorges University, Yichang, China.
Urol Int. 2018;100(1):25-30. doi: 10.1159/000479090. Epub 2017 Aug 16.
Escherichia coli O157 is an important food-borne pathogen that can cause diarrhoea, hemorrhagic colitis, and hemolytic uraemic syndrome. Enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR) and multilocus sequence typing (MLST) are good methods for molecular typing and the extensive use of antibiotics is a contributing factor to the increasing incidence of antimicrobial-resistant for these strains.
The aim of this study was to determine the prevalence, antimicrobial resistance, and genetic diversity of E. coli O157 based on the prevalence of urinary tract infection (UTI) in Hubei, China.
We obtained 23 (8.07%) E. coli O157 isolates from 285 UTI patients in Hubei, China. All isolates were subjected to antibiotic susceptibility analysis, and molecular typing was performed using ERIC-PCR and MLST. Antimicrobial susceptibility results indicated that most strains were resistant to penicillin (95.65%), chloramphenicol (73.91%), and ampicillin (69.57%). All isolates were discovered to be multiresistant (resistance to more than 3 antibiotics). Genetic variability analysis showed that all of the isolates were grouped into 4 clusters both by ERIC-PCR and MLST.
Our findings demonstrated the presence of E. coli O157 in UTIs, provided insights into the dissemination of antibiotic-resistant strains, and improved our knowledge of E. coli O157 risk assessment in UTIs.
大肠杆菌O157是一种重要的食源性病原体，可引起腹泻、出血性结肠炎和溶血性尿毒综合征。肠杆菌基因间重复一致序列聚合酶链反应（ERIC-PCR）和多位点序列分型（MLST）是分子分型的良好方法，而抗生素的广泛使用是这些菌株耐药性发生率增加的一个促成因素。
本研究旨在根据中国湖北尿路感染（UTI）的患病率，确定大肠杆菌O157的患病率、耐药性和遗传多样性。
我们从中国湖北的285例UTI患者中获得了23株（8.07%）大肠杆菌O157分离株。所有分离株均进行了药敏分析，并使用ERIC-PCR和MLST进行分子分型。药敏结果表明，大多数菌株对青霉素（95.65%）、氯霉素（73.91%）和氨苄西林（69.57%）耐药。所有分离株均被发现具有多重耐药性（对3种以上抗生素耐药）。遗传变异性分析表明，所有分离株通过ERIC-PCR和MLST均被分为4个簇。
我们的研究结果证明了UTI中存在大肠杆菌O157，深入了解了耐药菌株的传播情况，并提高了我们对UTI中大肠杆菌O157风险评估的认识。
