Klip A, Britt B A, Elliott M E, Pegg W, Frodis W, Scott E
Lancet. 1987 Feb 28;1(8531):463-6. doi: 10.1016/s0140-6736(87)92086-1.
The cytoplasmic concentration of ionised calcium, [Ca2+]i, is believed to be altered by agents that induce a malignant hyperthermia (MH) crisis in susceptible individuals. MH patients were identified by the halothane and halothane/caffeine contracture tests done in isolated muscle biopsy specimens. [Ca2+]i was measured in isolated peripheral blood mononuclear cells from MH patients and controls by means of the fluorescent calcium ion indicator quin2. In the absence of halothane there was no significant difference in [Ca2+]i in cells from normal and MH patients. Addition of halothane (4 microliter/ml) significantly increased [Ca2+]i in cells from MH patients but not in controls. The halothane-induced increase in [Ca2+]i required extracellular calcium ions. This is the first evidence of the mechanism of action of halothane in cells of MH patients; the differential effect of halothane on [Ca2+]i might constitute the basis for a non-invasive screening test for MH.
人们认为，诱导易感个体发生恶性高热（MH）危象的药物会改变细胞质中游离钙离子的浓度，即[Ca2+]i。通过对分离的肌肉活检标本进行氟烷和氟烷/咖啡因挛缩试验来识别MH患者。利用荧光钙离子指示剂喹啉2，对MH患者和对照组的分离外周血单个核细胞中的[Ca2+]i进行测量。在没有氟烷的情况下，正常人和MH患者细胞中的[Ca2+]i没有显著差异。添加氟烷（4微升/毫升）后，MH患者细胞中的[Ca2+]i显著增加，而对照组细胞中的[Ca2+]i没有增加。氟烷诱导的[Ca2+]i增加需要细胞外钙离子。这是氟烷在MH患者细胞中作用机制的首个证据；氟烷对[Ca2+]i的差异效应可能构成MH非侵入性筛查试验的基础。
