Fletcher J E, Conner G E, Allen G C, Huggins F J, Rosenberg H
Department of Anesthesiology Hahnemann University, Philadelphia.
Acta Anaesthesiol Scand. 1990 Nov;34(8):636-9. doi: 10.1111/j.1399-6576.1990.tb03162.x.
Anaesthetic-induced increases in cytoplasmic free Ca2+ have been reported to be greater in lymphocytes from malignant hyperthermia (MH) susceptible patients than in those from controls, suggesting that this may be the basis for a less invasive test for MH susceptibility. In the present study the cytoplasmic Ca2+ concentrations of lymphocytes were monitored with indo-1 in 14 control subjects (nine fasted and five nonfasted) and five fasted MH susceptible and three fasted nonsusceptible patients, diagnosed by the halothane and caffeine contracture tests. No relationship was observed between MH susceptibility and Ca2+ concentrations in lymphocytes in the absence or presence of halothane. There was, however, a relationship in control subjects between fasting and the response of lymphocytes to halothane, with the halothane-induced Ca2+ increase being considerably larger in nonfasted subjects.
据报道，麻醉诱导导致的恶性高热（MH）易感患者淋巴细胞胞浆游离钙离子增加幅度大于对照组，这表明这可能是一种侵入性较小的MH易感性检测方法的基础。在本研究中，通过氟烷和咖啡因挛缩试验诊断的14名对照受试者（9名禁食和5名非禁食）、5名禁食的MH易感患者和3名禁食的非易感患者，用indo-1监测淋巴细胞的胞浆钙离子浓度。在有无氟烷的情况下，均未观察到MH易感性与淋巴细胞钙离子浓度之间的关系。然而，在对照受试者中，禁食与淋巴细胞对氟烷的反应之间存在关系，非禁食受试者中氟烷诱导的钙离子增加幅度要大得多。
