Sun Songmei, Du Xing, Xu Mengxin, Liu Meijuan, Liu Zhifeng
Basic Medical Department, School of Pharmacy, Yantai University, Yantai, Shandong 264005, P.R. China.
Pharmacy Department, Yantai Yuhuangding Hospital, Yantai, Shandong 264000, P.R. China.
Exp Ther Med. 2017 Aug;14(2):1837-1845. doi: 10.3892/etm.2017.4679. Epub 2017 Jun 27.
The present study aimed to investigate the anti-exudative effects of sodium ferulate combined with oxymatrine in a mouse model of acetic acid-induced peritonitis. Furthermore, the underlying mechanisms were explored by determining the effects of these drugs on the volume and aquaporin 1 (AQP1) expression in vascular endothelial cells on omentum majus and human umbilical vein endothelial cells (HUVEC). Treatment with sodium ferulate combined with oxymatrine was shown to significantly inhibit acetic acid-induced vascular permeability in the peritonitis model mice and furthermore to significantly decrease the optical density of Evans blue, the leukocyte number and the levels of interleukin-6, C-reactive protein and interferon-γ in peritoneal lavage fluid. Pathological analysis of the omentum majus revealed that sodium ferulate and oxymatrine combination treatment significantly alleviated vascular endothelial cell edema and capillary loss. , flow cytometry revealed that the volume of HUVECs was significantly reduced in the drug treatment groups, as reflected in the forward scatter value. The optical density of AQP1 on the membrane of the vascular endothelial cells on omentum majus and HUVECs were significantly increased in the drug treatment groups compared with the model group. These results indicated that sodium ferulate and oxymatrine combination treatment possessed prominent anti-exudative effects and that the underlying mechanisms are likely to include the improvement of vascular endothelial cellular edema, possibly by upregulation of AQP1 expression on their membrane, which requires further exploration.
本研究旨在探讨阿魏酸钠联合氧化苦参碱在醋酸诱导的小鼠腹膜炎模型中的抗渗出作用。此外，通过测定这些药物对大网膜血管内皮细胞和人脐静脉内皮细胞（HUVEC）体积及水通道蛋白1（AQP1）表达的影响，探索其潜在机制。结果显示，阿魏酸钠联合氧化苦参碱治疗可显著抑制腹膜炎模型小鼠醋酸诱导的血管通透性，进而显著降低伊文思蓝的光密度、白细胞数量以及腹腔灌洗液中白细胞介素-6、C反应蛋白和干扰素-γ的水平。大网膜病理分析表明，阿魏酸钠与氧化苦参碱联合治疗可显著减轻血管内皮细胞水肿和毛细血管损失。此外，流式细胞术显示，药物治疗组HUVEC的体积显著减小，这在前向散射值中得到体现。与模型组相比，药物治疗组大网膜血管内皮细胞和HUVEC细胞膜上AQP1的光密度显著增加。这些结果表明，阿魏酸钠与氧化苦参碱联合治疗具有显著的抗渗出作用，其潜在机制可能包括改善血管内皮细胞水肿，可能是通过上调其细胞膜上AQP1的表达来实现的，这有待进一步探索。
