Li S B, Yang K S, Zhang Y T
Department of Orthopedics, Affiliated Hospital of Dali University, Dali, Yunnan Province, China.
Department of Orthopedics, Affiliated Hospital of Dali University, Dali, Yunnan Province, China
Genet Mol Res. 2014 Oct 8;13(4):8225-33. doi: 10.4238/2014.October.8.4.
Lumbar intervertebral disc degeneration is induced by multiple factors, but few studies have examined the effects of aquaporins on this process. We compared the expression levels of aquaporins 1 and 3 in normal and degenerative lumbar intervertebral discs. Fifteen normal and 15 degenerative lumbar intervertebral disc tissues were excised from lumbar burst fracture patients during orthopedic operations at the Dali College subsidiary hospital. Tissues were stained with hematoxylin-eosin and the expression levels of aquaporins 1 and 3 were measured by immunohistochemistry. Hematoxylin-eosin-staining results illustrated that the structures of the intervertebral disc tissues from the control group were clear, with distinct collagen fiber shapes and slight edema but without mucoid degeneration. Structures of the intervertebral disc tissues from the disease group were obscure and disordered with hyperplastic collagen fibers and tissues of severe inflammatory edemas with necrosis mucoid degeneration. Immunohistochemistry results demonstrated that the average absorbances of aquaporins 1 and 3 in the disease group were significantly lower than those in the control group (P < 0.01), suggesting that the reduction of aquaporins 1 and 3 may be a factor resulting in lumbar intervertebral disc degeneration.
腰椎间盘退变由多种因素引起，但很少有研究探讨水通道蛋白在这一过程中的作用。我们比较了正常和退变腰椎间盘中水通道蛋白1和3的表达水平。从大理学院附属医院骨科手术中的腰椎爆裂骨折患者身上切除15个正常和15个退变的腰椎间盘组织。组织用苏木精-伊红染色，并通过免疫组织化学法检测水通道蛋白1和3的表达水平。苏木精-伊红染色结果表明，对照组椎间盘组织结构清晰，胶原纤维形态明显，有轻微水肿但无黏液样变性。疾病组椎间盘组织结构模糊、紊乱，胶原纤维增生，组织有严重的炎性水肿伴坏死黏液样变性。免疫组织化学结果显示，疾病组水通道蛋白1和3的平均吸光度显著低于对照组（P < 0.01），提示水通道蛋白1和3的减少可能是导致腰椎间盘退变的一个因素。
