Naalsund L U, Fonnum F
Neurotoxicology. 1986 Fall;7(3):53-62.
The effects of trimethyltin (TMT) on high-affinity uptake, release and sodium-independent binding of glutamic acid and gamma-aminobutyric acid (GABA) were studied in vitro in homogenates of hippocampal tissue. TMT (50 micron) increased the release of glutamic acid from synaptosomes in the resting state (5 mM K+), whereas the release of GABA was only slightly affected. High affinity uptake of glutamate was inhibited by TMT in the same concentration range as release. The uptake of GABA was only affected by TMT-concentrations from 500 micron to 5 mM. The sodium independent binding of both glutamate and GABA, usually assumed to be binding to receptor sites, were inhibited with 50 microM or more TMT in the incubation medium. The results indicate that TMT can interfere with several different events of the neurotransmission process in the central nervous system at concentrations which can be obtained in the brain of rats after a sublethal dose of the compound.
在体外对海马组织匀浆进行研究，以探讨三甲基锡（TMT）对谷氨酸和γ-氨基丁酸（GABA）的高亲和力摄取、释放以及非钠依赖性结合的影响。TMT（50微摩尔）增加了静息状态（5毫摩尔钾离子）下突触体中谷氨酸的释放，而GABA的释放仅受到轻微影响。在与释放相同的浓度范围内，TMT抑制了谷氨酸的高亲和力摄取。GABA的摄取仅受500微摩尔至5毫摩尔浓度的TMT影响。通常认为与受体位点结合的谷氨酸和GABA的非钠依赖性结合，在孵育培养基中受到50微摩尔或更高浓度TMT的抑制。结果表明，在给予该化合物亚致死剂量后，大鼠脑内可达到的浓度下，TMT能够干扰中枢神经系统神经传递过程中的几种不同事件。
