Pharmaceutical Biology Department, Faculty of Pharmacy and Biotechnology, German University in Cairo, Cairo 11835, Egypt.
Biology Department, Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Im Neuenheimer Feld 364, Heidelberg 69120, Germany.
Molecules. 2019 Jul 19;24(14):2633. doi: 10.3390/molecules24142633.
(L.) Schott is a popular Asian tree widely used in traditional medicine. The current study explored the potential stress resistance and antioxidant activities of its fruits. Phytochemical profiling of the hydroalcoholic fruit extract was done via high performance liquid chromatography-photodiode array-electrospray ionization-mass/mass (HPLC-PDA-ESI-MS/MS). Twenty four phenolic constituents were tentatively identified in the extract. The () nematode model in addition to trimethyltin (TMT)-induced neurotoxicity mouse model were used for in vivo evaluation of its antioxidant properties. The ability of the extract to enhance stress resistance was manifested through increasing survival rate by 44.7% and decreasing basal reactive oxygen species (ROS) levels by 72.3% in . In addition, the extract increased the levels of the stress response enzyme superoxide dismutase-3 (Sod-3) by 55.5% and decreased the expression of heat shock protein-16.2 (Hsp-16.2) in nematodes, which had been challenged by juglone, by 21%. Using a mouse model, the extract significantly decreased the expression of the oxidative stress marker malondialdehyde (MDA). Furthermore, an elevation in the levels of the antioxidant marker glutathione (GSH), SOD and heme oxygenase-1 (HO-1) enzymes were observed. Our findings imply that has the potential to be used in future studies focusing on diseases associated with oxidative stress.
（L.）Schott 是一种广受欢迎的亚洲树种，广泛应用于传统医学。本研究探讨了其果实的潜在抗应激和抗氧化活性。采用高效液相色谱-光电二极管阵列-电喷雾电离-质谱/质谱联用技术（HPLC-PDA-ESI-MS/MS）对水醇提取物进行了植物化学成分分析。在提取物中初步鉴定出 24 种酚类成分。利用秀丽隐杆线虫模型和三甲基锡（TMT）诱导的神经毒性小鼠模型对其抗氧化性能进行了体内评价。提取物通过提高线虫的存活率（提高 44.7%）和降低基础活性氧（ROS）水平（降低 72.3%）来增强其抗应激能力。此外，提取物使应激反应酶超氧化物歧化酶-3（Sod-3）的水平增加了 55.5%，并使应激后线虫中热休克蛋白-16.2（Hsp-16.2）的表达降低了 21%。在小鼠模型中，提取物显著降低了氧化应激标志物丙二醛（MDA）的表达。此外，观察到抗氧化标志物谷胱甘肽（GSH）、SOD 和血红素加氧酶-1（HO-1）酶水平的升高。我们的研究结果表明，Schott 具有在未来与氧化应激相关疾病研究中应用的潜力。
