a Department of Microbiology and Molecular Biology , Brigham Young University , Provo UT.
b Department of Biology , Brigham Young University , Provo UT.
Cancer Biol Ther. 2018;19(10):913-920. doi: 10.1080/15384047.2017.1360453. Epub 2018 May 10.
Classical anti-inflammatory cytokines are known to play a role in both cancer progression as well as cancer elimination. We evaluated the anti-inflammatory cytokines IL-10 and TGF-β in patients with colon adenocarcinoma and metastatic colon adenocarcinoma using immunohistochemical assays to determine the expression of the cytokines between various malignant tissues. We found tissues stained with TGF-β showed no significant upregulation within malignant tumors when compared with normal tissue controls. We observed high levels of TGF-β presence in most tissues similar to GAPDH expression. Within both colon adenocarcinoma and metastatic carcinomas there was a significant variability among patients in the expression of IL-10. While some patients experienced insignificant increases in the cytokine compared with controls, other patients had a clear upregulation of the protein within their tissue. In addition, there was an increase in the number of patients positive for IL-10 upregulation within metastatic tumors when compared with primary tumors. These data indicate that there is substantial variability between patients in regards to IL-10 expression, which may further aid in characterizing tumors and evaluating metastatic potential.
经典的抗炎细胞因子被认为在癌症的进展和消除中都发挥作用。我们使用免疫组织化学检测评估了结肠癌和转移性结肠癌患者中的抗炎细胞因子 IL-10 和 TGF-β，以确定细胞因子在各种恶性组织之间的表达。我们发现，与正常组织对照相比，TGF-β 染色的组织在恶性肿瘤中没有明显上调。我们观察到 TGF-β 在大多数组织中的存在水平与 GAPDH 表达相似。在结肠癌和转移性癌中，IL-10 的表达在患者之间存在显著差异。虽然一些患者与对照相比细胞因子水平没有显著增加，但其他患者组织中蛋白明显上调。此外，与原发性肿瘤相比，转移性肿瘤中 IL-10 上调的患者数量增加。这些数据表明，患者之间在 IL-10 表达方面存在很大差异，这可能进一步有助于肿瘤特征的描述和转移潜能的评估。
