Gossler Vanessa S A, Santos Francislaine A G, Azevedo Aline R DE, Gonçalves Priscila C, Rigolo Hugo A, Trevisan Cleonice, Masseno Ana Paula B, Cardoso Ana Paula M M, Papa Paula C, Castilho Caliê, Giometti Ines C
Faculdade de Ciências Agrárias, Universidade do Oeste Paulista/UNOESTE, Campus II, Rod. Raposo Tavares, Km 572, Limoeiro, 19067-175 Presidente Prudente, SP, Brazil.
Setor de Anatomia, Departamento de Cirurgia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo/USP, Av. Prof. Dr. Orlando Marques Paiva, 87, 05508-270 São Paulo, SP, Brazil.
An Acad Bras Cienc. 2017 Jul-Sep;89(3):1719-1727. doi: 10.1590/0001-3765201720150611. Epub 2017 Aug 14.
This study aimed to determine the histological features of the endometrium of bitches, as well as the cell proliferation at specific moments of diestrus, 10, 20, 30, 40, 50 and 60 days post ovulation, correlating the endometrial thickness with the uterine cell proliferation and the metabolic state (weight, blood glucose and plasma cholesterol) of the animals. Therefore, the right and left uterine horns of 26 clinically healthy bitches submitted to ovariohysterectomy were histologically analyzed 10, 20, 30, 40, 50 and 60 days post ovulation. The hematoxylin-eosin and AgNOR staining techniques were performed. All parameters were evaluated by ANOVA and post-hoc Tukey test (p<0.05). The correlation between endometrial thickness and uterine cell proliferation, weight, blood glucose and plasma cholesterol of animals was observed using the Pearson method (p<0.05). In the present study, it is concluded that endometrial thickness does not differ at any of the moments analyzed in diestrus. The endometrial thickness is not influenced by hormones, weight, blood glucose or serum cholesterol of bitches in this phase of the estrous cycle. However, there is greater cell proliferation in the endometrium at day 40 compared to day 60 post ovulation under the influence of the endocrine profile.
本研究旨在确定母犬子宫内膜的组织学特征，以及发情后期排卵后10、20、30、40、50和60天特定时刻的细胞增殖情况，将子宫内膜厚度与子宫细胞增殖以及动物的代谢状态（体重、血糖和血浆胆固醇）相关联。因此，对26只接受卵巢子宫切除术的临床健康母犬在排卵后10、20、30、40、50和60天的左右子宫角进行了组织学分析。采用苏木精-伊红染色和AgNOR染色技术。所有参数均通过方差分析和事后Tukey检验进行评估（p<0.05）。使用Pearson方法观察子宫内膜厚度与动物子宫细胞增殖、体重、血糖和血浆胆固醇之间的相关性（p<0.05）。在本研究中，得出的结论是，发情后期分析的任何时刻子宫内膜厚度均无差异。在发情周期的这个阶段，母犬的子宫内膜厚度不受激素、体重、血糖或血清胆固醇的影响。然而，在内分泌状态的影响下，排卵后40天子宫内膜中的细胞增殖比60天更多。
