Universidade Federal de São João del-Rei - Departamento de Medicina - Laboratório de Patologia Experimental - Divinópolis (MG) - Brazil.
Universidade Federal de Minas Gerais - Faculdade de Farmácia - Belo Horizonte (MG) - Brazil.
Acta Cir Bras. 2024 Jul 29;39:e393924. doi: 10.1590/acb393924. eCollection 2024.
This study aimed to evaluate the therapeutic effect of an ethanol extract of Ocimum basilicum L. (EEOb) aerial parts against Ehrlich's experimental tumor (EET) in mice.
Swiss mice were divided into two groups (control and treated; n = 6). On day 21, all mice were inoculated subcutaneously with 2 × 106 (0.05 mL) EET cells in the left paw for solid tumor development. This study lasted 28 days. Treatment began 24 hours after inoculation with EET. Measurements of dorsoplantar thickness were used to assess tumor growth. The paw pad was collected for histopathological analysis and stained using the argyrophilic nucleolar organizing regions (AgNOR) technique and immunohistochemistry for proliferating cell nuclear antigen, Bcl-2 and Bax.
The treatment of animals with EEOb at 100 mg/kg intraperitoneally was able to reduce the growth (Control = 3.7 ± 0.1 mm vs. EEOb = 5.7 ± 0.2 mm) and the number of AgNORs of solid Ehrlich tumor. The antitumor effect of EEOb was associated with the induction of apoptosis of tumoral cell, as suggested by the reduction of the content of Bcl-2 induced by extract.
The study demonstrated that daily administration of EEOb is able to reduce the growth of EET by induce apoptosis of tumoral cells.
本研究旨在评估罗勒（Ocimum basilicum L.）地上部分乙醇提取物（EEOb）对艾氏腹水瘤（EET）在小鼠体内的治疗效果。
将瑞士小鼠分为两组（对照组和治疗组；n=6）。第 21 天，所有小鼠左后脚掌皮下接种 2×106（0.05 mL）EET 细胞，以建立实体瘤。本研究持续 28 天。接种 EET 后 24 小时开始治疗。测量背-跖厚度以评估肿瘤生长情况。采集脚掌垫进行组织病理学分析，并使用银染核仁形成区（AgNOR）技术和增殖细胞核抗原、Bcl-2 和 Bax 的免疫组织化学染色进行染色。
腹腔内给予 100mg/kg EEOb 的动物治疗能够减少肿瘤的生长（对照组=3.7±0.1mm vs. EEOb=5.7±0.2mm）和 AgNOR 数量。EEOb 的抗肿瘤作用与诱导肿瘤细胞凋亡有关，这可从提取物诱导的 Bcl-2 含量减少得到证实。
本研究表明，每日给予 EEOb 能够通过诱导肿瘤细胞凋亡来减少 EET 的生长。
