Yam P Y, Petz L D, Knowlton R G, Wallace R B, Stock A D, de Lange G, Brown V A, Donis-Keller H, Blume K G
Transplantation. 1987 Mar;43(3):399-407. doi: 10.1097/00007890-198703000-00016.
We have studied the feasibility of using DNA restriction fragment-length polymorphisms (RFLP) to study marrow engraftment in 27 patients after allogeneic bone marrow transplantation, and have compared these results with those obtained using red blood cell antigens, cytogenetics, and immunoglobulin allotypes. Using highly polymorphic DNA probes, we have documented stable chronic mixed hematopoietic chimerism, have identified transient mixed chimeras, have excluded mixed chimerism with high probability in retrospective studies even when a pretransplant DNA sample was not available, have documented marrow engraftment in the early posttransplant period, and have studied the origin of leukemic cells in patients with recurrent disease. We have evaluated the advantages and disadvantages of several genetic markers and have developed tentative statements concerning the prognosis of patients with mixed chimerism. We conclude that DNA RFLP are powerful and practical genetic markers in bone marrow transplantation studies and that further studies of mixed hematopoietic chimerism are warranted.
我们研究了使用DNA限制性片段长度多态性（RFLP）来研究27例异基因骨髓移植患者骨髓植入情况的可行性，并将这些结果与使用红细胞抗原、细胞遗传学和免疫球蛋白同种异型所获得的结果进行了比较。使用高度多态性的DNA探针，我们记录了稳定的慢性混合造血嵌合体，识别出了短暂性混合嵌合体，在回顾性研究中即使没有移植前的DNA样本也能以高概率排除混合嵌合体，记录了移植后早期的骨髓植入情况，并研究了复发患者白血病细胞的起源。我们评估了几种遗传标记的优缺点，并就混合嵌合体患者的预后提出了初步观点。我们得出结论，DNA RFLP在骨髓移植研究中是强大而实用的遗传标记，有必要对混合造血嵌合体进行进一步研究。
