Huijbers Ivo J
Mouse Clinic for Cancer and Aging, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands.
Methods Mol Biol. 2017;1642:1-19. doi: 10.1007/978-1-4939-7169-5_1.
The generation of a new genetically modified mouse strain is a big hurdle to take for many researchers. It is often unclear which steps and decisions have to be made prior to obtaining the desired mouse model. This review aims to help researchers by providing a decision guide that answers the essential questions that need to be asked before generating the most suitable genetically modified mouse line in the most optimal timeframe. The review includes the latest technologies in both the stem cell culture and gene editing tools, particularly CRISPR/Cas9, and provides compatibility guidelines for selecting among the different types of genetic modifications that can be introduced in the mouse genome and the various routes for introducing these modifications into the mouse germline.
对于许多研究人员来说，培育一种新的转基因小鼠品系是一个巨大的障碍。在获得所需的小鼠模型之前，通常不清楚需要采取哪些步骤以及做出哪些决策。本综述旨在通过提供一份决策指南来帮助研究人员，该指南回答了在最理想的时间范围内培育出最合适的转基因小鼠品系之前需要提出的基本问题。该综述涵盖了干细胞培养和基因编辑工具（特别是CRISPR/Cas9）方面的最新技术，并提供了兼容性指导方针，用于在可引入小鼠基因组的不同类型基因修饰以及将这些修饰引入小鼠种系的各种途径中进行选择。
