Phillips A G, Jakubovic A, Fibiger H C
Brain Res. 1987 Jan 27;402(1):109-16. doi: 10.1016/0006-8993(87)91053-5.
Changes in the activity of dopaminergic neurons associated with intracranial self-stimulation of the ventral tegmentum were assessed by measuring the accumulation of 3,4-dihydroxyphenylalanine (DOPA) after inhibition of aromatic amino acid decarboxylase by NSD-1015. When compared to implanted unstimulated controls, DOPA concentrations were elevated significantly in the nucleus accumbens, striatum and olfactory tubercle in the hemisphere ipsilateral to the electrode, after a 30 min session of self-stimulation. The concentration of DOPA in the contralateral nucleus accumbens and striatum did not differ from control levels, although relative to control values it was significantly increased in the contralateral olfactory tubercle. A similar analysis of in vivo tyrosine hydroxylase activity in these brain regions following a 30 min session of lever pressing for food reward on a fixed-ratio (FR-8) schedule failed to reveal any significant changes relative to control subjects. These results are consistent with a role for dopamine in brain-stimulation reward obtained from electrical stimulation of the ventral tegmental area but do not provide evidence for dopaminergic mediation of the rewarding properties of food.
通过测量NSD - 1015抑制芳香族氨基酸脱羧酶后3,4 - 二羟基苯丙氨酸（DOPA）的积累，评估与腹侧被盖区颅内自我刺激相关的多巴胺能神经元活动的变化。与植入未受刺激的对照组相比，在30分钟的自我刺激后，电极同侧半球的伏隔核、纹状体和嗅结节中的DOPA浓度显著升高。对侧伏隔核和纹状体中的DOPA浓度与对照水平无差异，尽管相对于对照值，对侧嗅结节中的DOPA浓度显著增加。在固定比率（FR - 8）时间表上进行30分钟杠杆按压以获取食物奖励后，对这些脑区体内酪氨酸羟化酶活性进行的类似分析未能发现相对于对照受试者有任何显著变化。这些结果与多巴胺在通过腹侧被盖区电刺激获得的脑刺激奖励中所起的作用一致，但没有为食物奖励特性的多巴胺能介导提供证据。
