Zhang Jingjing, Zhi Chunchun, Zhen Fuxi, Yuan Xiaoqin, Jiao Chunhua, Zhu Hong, Zhu Hui, Feng Yadong
State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Jiangning, Nanjing, China.
Department of Prenatal Diagnosis, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing, China.
Proteomics Clin Appl. 2017 Dec;11(11-12). doi: 10.1002/prca.201600167. Epub 2017 Sep 25.
Esophageal squamous cell carcinoma (ESCC) is one of the most common cancers worldwide and is the fourth most lethal cancer in China. Little is known about the proteome of high grade esophageal squamous intraepithelial neoplasia (HGN), which is a premalignant lesion of ESCC. A quantitative proteomic analysis using an isobaric tag for relative and absolute quantification (iTRAQ) approach is used to characterize the protein expression profiles in HGN. Among the 3156 identified proteins, a total of 236 proteins are discovered to be differentially expressed. Compared with paired normal esophageal epithelial tissues, 138 proteins are upregulated and 98 proteins are downregulated in HGN. Bioinformatics analyses are performed according to gene ontology, clusters of orthologous groups, and kyoto encyclopedia of genes and genomes enrichment analyses. Six differentially expressed proteins are chosen and validated by Western blotting. The results of the study increase our understanding of early tumorigenesis during ESCC, and provide insights into the proteome at the initial stages of the disease that can be used to identify potential biomarkers for early diagnosis and for therapeutic targets.
食管鳞状细胞癌（ESCC）是全球最常见的癌症之一，在中国是致死率第四高的癌症。人们对高级别食管鳞状上皮内瘤变（HGN）的蛋白质组了解甚少，而HGN是ESCC的一种癌前病变。使用等压标签相对和绝对定量（iTRAQ）方法进行定量蛋白质组分析，以表征HGN中的蛋白质表达谱。在鉴定出的3156种蛋白质中，共发现236种蛋白质存在差异表达。与配对的正常食管上皮组织相比，HGN中有138种蛋白质上调，98种蛋白质下调。根据基因本体论、直系同源簇和京都基因与基因组百科全书富集分析进行生物信息学分析。选择六种差异表达的蛋白质并通过蛋白质印迹法进行验证。该研究结果增进了我们对ESCC早期肿瘤发生的理解，并为疾病初始阶段的蛋白质组提供了见解，可用于识别早期诊断的潜在生物标志物和治疗靶点。
